@article{mbs:/content/journal/micro/10.1099/00221287-128-12-2841, author = "Fry, John C. and Zia, Talat", title = "Viability of Heterotrophic Bacteria in Freshwater", journal= "Microbiology", year = "1982", volume = "128", number = "12", pages = "2841-2850", doi = "https://doi.org/10.1099/00221287-128-12-2841", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-12-2841", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The viabilities of planktonic bacteria from nine freshwaters and one raw sewage were compared. Viabilities estimated from microcolony formation in slide culture (18·4–48·7%) were higher than a conventional plate count method (1·8–14·9%). Microcolony viability was estimated in four ways; the one recommended was the ratio of the microcolony count and total count of microcolonies and single cells after 24 or 72 h incubation. Bacterial viability strongly correlated with the percentage of bacteria <0·2 μm3 in size and the concentration of soluble carbohydrate in the water.", }