RT Journal Article SR Electronic(1) A1 Liu, H-M. A1 Chak, K. F. A1 Piggot, P. J.YR 1982 T1 Isolation and Characterization of a Recombinant Plasmid Carrying a Functional Part of the Bacillus subtilis spoIIA Locus JF Microbiology, VO 128 IS 11 SP 2805 OP 2812 DO https://doi.org/10.1099/00221287-128-11-2805 PB Microbiology Society, SN 1465-2080, AB Plasmid pHM2 consists of a 3ยท3 kb insert of Bacillus subtilis DNA in the chimeric plasmid pHV33, and can replicate in Escherichia coli and B. subtilis. In B. subtilis, pHM2 complements the defects resulting from mutations spo-42, spo-50, spo-69 and sas-1 in the spoIIA locus. This complementation can occur in recE4 strains where recombination of the plasmid with the chromosome is prevented, and the chromosome retains the mutant allele. Thus the plasmid carries a functional part of the spoIIA locus; it does not contain the complete locus as it cannot complement several other spoIIA mutations. It is likely that the locus is complex, containing at least two genes., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-11-2805