1887

Abstract

Plasmid pHM2 consists of a 3·3 kb insert of DNA in the chimeric plasmid pHV33, and can replicate in and . In , pHM2 complements the defects resulting from mutations and in the locus. This complementation can occur in strains where recombination of the plasmid with the chromosome is prevented, and the chromosome retains the mutant allele. Thus the plasmid carries a functional part of the locus; it does not contain the complete locus as it cannot complement several other mutations. It is likely that the locus is complex, containing at least two genes.

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1982-11-01
2021-07-23
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