1887

Abstract

Plasmid pHM2 consists of a 3·3 kb insert of DNA in the chimeric plasmid pHV33, and can replicate in and . In , pHM2 complements the defects resulting from mutations and in the locus. This complementation can occur in strains where recombination of the plasmid with the chromosome is prevented, and the chromosome retains the mutant allele. Thus the plasmid carries a functional part of the locus; it does not contain the complete locus as it cannot complement several other mutations. It is likely that the locus is complex, containing at least two genes.

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1982-11-01
2024-12-12
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References

  1. Bott K. F., Wilson G. A. 1968; Metabolic and nutritional factors influencing the development of competence for transfection of Bacillus subtilis. Bacteriological Reviews 32:370–378
    [Google Scholar]
  2. Brown M. G. M., Weston A., Saunders J. R., Humphreys G. O. 1979; Transformation of Escherichia coli C600 by plasmid DNA at different phases of growth. FEMS Microbiology Letters 5:219–222
    [Google Scholar]
  3. Canosi U., Morelli G., Trautner T. A. 1978; The relationship between molecular structure and transformation efficiency of some S. aureus plasmids isolated from B. subtilis. Molecular and General Genetics 166:259–267
    [Google Scholar]
  4. Coote J. G. 1972; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  5. Csonka L. N., Clark A. J. 1980; Construction of an Hfr strain useful for transferring recA mutations between Escherichia coli strains. Journal of Bacteriology 143:529–530
    [Google Scholar]
  6. Daniels D. L., Dewet J. R., Blattner F. R. 1980; New map of bacteriophage lambda DNA. Journal of Virology 33:390–400
    [Google Scholar]
  7. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Ehrlich S. D. 1978; DNA cloning in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America 751433–1436
    [Google Scholar]
  9. Ehrlich S. D., Niaudet B., Michel B. 1981; Use of plasmid from Staphylococcus aureus for cloning of DNA in Bacillus subtilis. Current Topics in Microbiology and Immunology 96:19–29
    [Google Scholar]
  10. Giles K. W., Myers A. 1965; An improved diphenylamine method for the estimation of deoxy-ribonucleic acid. Nature; London: 206:93
    [Google Scholar]
  11. Gryczan T. J., Contente S., Dubnau D. 1978; Characterisation of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. Journal of Bacteriology 134:318–329
    [Google Scholar]
  12. Guerry P., Le Blanc D. J., Falkow S. 1973; General method for the isolation of deoxyribonucleic acid. Journal of Bacteriology 116:1064–1066
    [Google Scholar]
  13. Hoch J. A. 1971; Selection of cells transformed to prototrophy for sporulation markers. Journal of Bacteriology 105:1200–1201
    [Google Scholar]
  14. Ionesco H., Schaeffer P. 1968; Localisation chromosomique de certains mutants asporogenes de Bacillus subtilis Marburg. Annales de l’Institut Pasteur 114:11–20
    [Google Scholar]
  15. Jayaraman K., Keryer K., Szulmajster J. 1981; Cloning of a fragment of B. subtilis DNA carrying the spoOB and pheA genes. FEMS Microbiology Letters 10:273–276
    [Google Scholar]
  16. Karn J., Brenner S., Barnett L., Cesareni G. 1980; Novel bacteriophage λ cloning vector. Proceedings of the National Academy of Sciences of the United States of America 775172–5176
    [Google Scholar]
  17. Kawamura F., Shimotsu H., Saito H., Hirochika H., Kobayashi Y. 1981; Cloning of spoO genes with bacteriophage and plasmid vectors in Bacillus subtilis. In Sporulation and Germination pp. 109–113 Edited by Levinson H. S., Sonenshein A. L., Tipper D. J. Washington, D.C: American Society for Microbiology;
    [Google Scholar]
  18. Klein R. D., Selsing E., Wells R. D. 1980; A rapid microscale technique for isolation of recombinant plasmid DNA suitable for restriction enzyme analysis. Plasmid 3:88–91
    [Google Scholar]
  19. Kreft J., Hughes C. 1981; Cloning vectors derived from plasmids and phage of Bacillus. Current Topics in Microbiology and Immunology 96:1–17
    [Google Scholar]
  20. Mazza G., Galizzi A. 1978; The genetics of DNA replication, repair and recombination in Bacillus subtilis. Microbiologica 1:111–135
    [Google Scholar]
  21. Miller J. H. 1972 Experiments in Molecular Genetics New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Parker R. C., Seed B. 1980; Two-dimensional agarose gel electrophoresis. ‘Sea-plaque’ agarose dimension. Methods in Enzymology 65:358–363
    [Google Scholar]
  23. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis : a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  24. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  25. Piggot P. J., Taylor S. Y. 1977; New types of mutation affecting formation of alkaline phosphatase by Bacillus subtilis in sporulation conditions. Journal of General Microbiology 102:69–80
    [Google Scholar]
  26. Primrose S. B., Ehrlich S. D. 1981; Isolation of plasmid deletion mutants and study of their instability. Plasmid 6:193–201
    [Google Scholar]
  27. Rapoport G., Klier A., Billault A., Fargette F., Dedonder R. 1979; Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis. Molecular and General Genetics 176:239–245
    [Google Scholar]
  28. Rouyard J-F., Ionesco H., Schaeffer P. 1967; Classification genetique de certains mutants de sporulation de Bacillus subtilis Marburg. Annales de l’Institut Pasteur 113:675–683
    [Google Scholar]
  29. Sutcliffe J. G. 1978; pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acid Research 5:2721–2728
    [Google Scholar]
  30. Ullman A., Perrin D. 1970; Complementation in β-galactosidase. In The Lactose Operon pp. 143–172 Edited by Beckwith J. R., Zipser D. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Yudkin M. D., Turley L. 1980; Suppression of asporogeny in Bacillus subtilis. Allele-specific suppression of a mutation in the spoIIA locus. Journal of General Microbiology 121:69–78
    [Google Scholar]
  32. Yudkin M. D., Turley L. 1981; Mapping of six mutations in the spoil A locus of Bacillus subtilis and studies of their response to a nonsense suppressor. Journal of General Microbiology 124:255–261
    [Google Scholar]
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