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Volume 128, Issue 11

Phages Iα and I-2: IncI Plasmid-dependent Bacteriophages Free

Abstract

Phage Iα was isolated from sewage from Windhoek, South West Africa. It formed relatively clear plaques about 2 mm in diameter, on sensitive strains of K12 and LT2. The phage had an hexagonal outline with a diameter of about 24 nm, contained RNA and was resistant to chloroform. Phage Iα formed plaques or propagated only on organisms carrying I plasmids or the Iγ plasmid R621a. The efficiency of plating was higher on than on hosts. The phage adsorbed along the length of shafts of I pili.

Phage I-2 was isolated from Pretoria sewage. It was a filamentous virus and individual virions varied considerably in length. Phage I-2 formed turbid plaques which varied from pin point to about 1 mm in diameter on all hosts. It was resistant to RNAase and sensitive to chloroform. Phage I-2 had a spectrum of activity limited to strains harbouring I plasmids but the adsorption site could not be demonstrated. The phage was not related serologically to phages If1 or PR64FS.

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  • Revised:
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© Society for General Microbiology 1982
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1982-11-01
2025-04-25
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Phages Iα and I2-2: IncI Plasmid-dependent Bacteriophages
Microbiology 128, 2797 (1982); https://doi.org/10.1099/00221287-128-11-2797
/content/journal/micro/10.1099/00221287-128-11-2797
/content/journal/micro/10.1099/00221287-128-11-2797
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