1887

Abstract

Tyrosine aminotransferase, induced in by -tyrosine, was isolated from the culture medium, partially purified and characterized. The enzyme was inducible by both - and -tyrosine, although -tyrosine was the better inducer. The enzyme required -tyrosine and a-ketoglutarate as amino donor and amino acceptor, respectively. Its pH optimum in 50 m-potassium phosphate buffer was 7·6, the apparent Michaelis constants were 0·5 m for -tyrosine and 0·25 m for a-ketoglutarate; it had a molecular weight of 110000.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2735
1982-11-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2735.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2735&mimeType=html&fmt=ahah

References

  1. Ainsworth G. C. 1971 Ainsworth and Bisby’s Dictionary of the Fungi, 6th edn. Kew, Surrey: Commonwealth Mycological Institute;
    [Google Scholar]
  2. Bekker Z. E. 1963 Fiziologia Gribov i ichpraktiches-koye Ispolzovaniye pp. 72–73 Moscow: Moscow University Press;
    [Google Scholar]
  3. Canellakis Z. N., Cohen P. P. 1956; Purification studies of tyrosine-α-ketoglutaric acid transaminase. Journal of Biological Chemistry 222:53–62
    [Google Scholar]
  4. Clements F. E., Shear C. L. 1973 The Genera of Fungi New York: Hafner Publishing Co;
    [Google Scholar]
  5. Diamondstone T. T. 1966; Assay of tyrosine transaminase activity by conversion of p-hydroxyphenyl pyruvate to p-hydroxybenzaldehyde. Analytical Biochemistry 16:395–401
    [Google Scholar]
  6. Eze L. C., Echetebu C. O. 1980; Some properties of aspartate and alanine aminotransferases from Trichodermaviride. Journal of General Microbiology 120:523–527
    [Google Scholar]
  7. Fries L. 1953; Factors promoting growth of Coprinusfimetarius (L) under high temperature conditions. Physiologiaplantarum 6:551–563
    [Google Scholar]
  8. Granner D. K., Tomkins G. M. 1970; Tyrosine aminotransferase (rat liver). Methods in Enzymology 17:633
    [Google Scholar]
  9. Hargrove J., Diesterhaft M., Noguchi T., Granner D. K. 1980; Identification of native tyrosine aminotransferase and explanation for the multiple forms. Journal of Biological Chemistry 255, (1):71–78
    [Google Scholar]
  10. Iwasaki Y., Laman C., Danenberg K., Pitot H. C. 1973; Studies on the induction and repression of enzymes in rat liver: characterization and metabolic regulation of multiple forms of tyrosine aminotransferase. European Journal of Biochemistry 34:347–357
    [Google Scholar]
  11. Kenny F. T. 1959; Properties of partially purified tyrosine-α-ketoglutarate transaminase from rat liver. Journal of Biological Chemistry 234:2707–2712
    [Google Scholar]
  12. Kenny F. I. 1962; Induction of tyrosine-α-ketoglutarate transaminase in rat liver. II. Enzyme purification and preparation of antitransaminase. Journal of Biological Chemistry 237:1605–1609
    [Google Scholar]
  13. Lin E. C. C., Knox W. E. 1958; Specificity of the adaptive response of tyrosine-a-ketoglutarate transaminase in rat. Journal of Biological Chemistry 233:1186–1189
    [Google Scholar]
  14. Ohisalo J. J., Pispa J. P. 1976; Heterogeneity of hepatic tyrosine-aminotransferase: separation of the multiple forms from rat and frog by isoelectric focussing and hydroxylapatite column chromatography and their partial characterization. Actachemica scandinavica B30:491–500
    [Google Scholar]
  15. Ohisalo J. J., Andersson S. M., Pispa J. P. 1977; Partial purification and properties of frog liver tyrosine aminotransferase. Biochemical Journal 163:411–417
    [Google Scholar]
  16. Rudman D., Meister A. 1953; Transamination in Escherichia coli. Journal of Biological Chemistry 200:591–604
    [Google Scholar]
  17. Valeriote F. A., Aurichio F., Tomkins G. M., Riley D. 1969; Purification and properties of rat liver tyrosine aminotransferase. Journal of Biological Chemistry 244, (13):3618–3624
    [Google Scholar]
  18. Whitaker J. R. 1963; Determination of molecular weights of proteins by gel filtration on Sephadex. Analytical Chemistry 35:1950–1953
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-11-2735
Loading
/content/journal/micro/10.1099/00221287-128-11-2735
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error