1887

Abstract

The inhibitory action of adenosine on the initiation of aggregation centre formation in starving was examined and used to test assumptions of theoretical models of cyclic AMP signalling. Adenosine at 5 m strongly inhibited aggregation centre initiation with-out preventing signal relay. Inosine or AMP at 10 m had no effect and did not prevent the effect of adenosine. Adenosine had no effect on cellular phosphodiesterase activity, contact site A formation, or chemotaxis to small drops of cyclic AMP. The chemotactically-related responses of rapid cyclic GMP formation and changes in light scattering after pulses of cyclic AMP did not show any inhibition or decrease in sensitivity to the signal in the presence of adenosine. However, binding of H-labelled cyclic AMP to cell surface receptors was strongly inhibited by adenosine. The maximum inhibition was approximately 90% and was non-competitive with respect to cyclic AMP. The role of cell surface cyclic AMP receptors in the mechanism of initiation and the implications of the effects of adenosine for the role of cyclic GMP as a secondary messenger are discussed.

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1982-11-01
2024-04-20
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References

  1. Beug H., Katz F. E., Gerisch G. 1973; Dynamics of antigen membrane sites relating to cell aggregation in Dictyostelium discoideum. Journal of Cell Biology 56:647–658
    [Google Scholar]
  2. Bradley S. G., Sussman M., Ennis H. L. 1956; Environmental factors affecting the aggregation of the cellular slime mold Dictyostelium discoideum. Journal of Protozoology 3:33–38
    [Google Scholar]
  3. Coukell M. B., Chan F. K. 1980; Precocious appearence and activation of an adenylate cyclase in a rapid developing mutant of Dictyostelium discoideum. FEBS Letters 110:39–42
    [Google Scholar]
  4. Darmon M., Brachet P. 1978; Chemotaxis and differentiation during aggregation of Dictyostelium discoideum amoebae. In Receptors and Recognition B5 Taxis and Behaviour pp. 101–139 Hazelbauer G. L. Edited by London: Chapman & Hall;
    [Google Scholar]
  5. Darmon M., Barrand P., Brachet P., Klein C., Pereira DA SILVA L. 1977; Phenotypic suppression of morphogenetic mutants of Dictyostelium discoideum. Developmental Biology 58:124–184
    [Google Scholar]
  6. Devreotes P. N., Derstine P. L., Steck T. L. 1979; Cyclic 3´,5´-AMP in Dictyostelium discoideum. Journal of Cell Biology 80:291–299
    [Google Scholar]
  7. Durston A. J. 1973; Dictyostelium discoideum aggregation fields as excitable media. Journal of Theoretical Biology 42:483–504
    [Google Scholar]
  8. Gerisch G., Hess B. 1974; Cyclic AMP controlled oscillations in suspended Dictyostelium cells. Their relation to morphogenetic cell interaction. Proceedings of the National Academy of Sciences of the United States of America 712118–2122
    [Google Scholar]
  9. Gerisch G., Fromm H., Huesgen A., Wick U. 1975; Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cells. Nature; London: 255:547–549
    [Google Scholar]
  10. Glazer P. M., Newell P. C. 1981; Initiation of aggregation by Dictyostelium discoideum in mutant populations lacking pulsatile signalling. Journal of General Microbiology 125:221–232
    [Google Scholar]
  11. Goldbeter A., Erneaux T. 1978; Excitability in adenylate-cyclase reaction in Dictyostelium discoideum. FEBS tetters 89:237–241
    [Google Scholar]
  12. Goldbeter A., Segel L. A. 1980; Control of developmental transitions in the cyclic AMP signalling system of Dictyostelium discoideum. Differentiation 17:127–135
    [Google Scholar]
  13. Green A. A., Newell P. C. 1975; Evidence for the existence of two types of cyclic AMP binding sites in aggregating cells of Dictyostelium discoideum. Cell 6:129–136
    [Google Scholar]
  14. Gross J. D., Peacey M. J., Trevan D. J. 1976; Signal emission and signal propagation during early aggregation in Dictyostelium discoideum. Journal of Cell Science 22:645–656
    [Google Scholar]
  15. Keller E. R., Segel L. A. 1970; Initiation of slime mold aggregation viewed as an instability. Journal of Theoretical Biology 26:399–415
    [Google Scholar]
  16. Mato J. M., Malchow D. 1978; Guanylate cyclase activation in response to chemotactic stimulation in Dictyostelium discoideum. FEBS tetters 90:119–122
    [Google Scholar]
  17. Mato J. M., Van Haastert P. J., Krens F. A., Rhijnsburger E. H., Dobbe F.C.P.M., Konijn T. M. 1977a; Cyclic AMP and folic acid mediated cyclic GMP accumulation in Dictyostelium discoideum. FEBS tetters 79:331–336
    [Google Scholar]
  18. Mato J. M., Krens F. A., Van Haastert P. J., Konijn T. M. 1977b; Unified control of chemotaxis and cyclic AMP-mediated cyclic GMP accumulation by cyclic AMP in Dictyostelium discoideum. Biochemical and Biophysical Research Communications 77:399–402
    [Google Scholar]
  19. Mosses D., Williams K. L., Newell P. C. 1975; The use of mitotic crossing-over for genetic analysis in Dictyostelium discoideum: mapping of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  20. Mullens I. A., Newell P. C. 1978; Cyclic AMP binding to cell surface receptors of Dictyostelium. Differentiation 10:171–176
    [Google Scholar]
  21. Newell P. C. 1977; Aggregation and cell surface receptors in cellular slime moulds. In Receptors and Recognition B3 Microbial Interactions, pp. 157 Reissig J. L. Edited by London: Chapman & Hall;
    [Google Scholar]
  22. Newell P. C. 1978; Cellular communication during aggregation of Dictyostelium. Journal of General Microbiology 104:1–13
    [Google Scholar]
  23. Newell P. C. 1982; Cell surface binding of adenosine to Dictyostelium discoideum and inhibition of pulsatile signalling. FEMS Microbiology tetters 13:417–421
    [Google Scholar]
  24. Ratner D. I., Newell P. C. 1978; Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. Journal of General Microbiology 109:225–236
    [Google Scholar]
  25. Ross F. M., Newell P. C. 1979; Genetics of aggregation pattern mutations in the cellular slime mould Dictyostelium discoideum. Journal of General Microbiology 115:289–300
    [Google Scholar]
  26. Ross F. M., Newell P. C. 1981; Streamers: chemotactic mutants of Dictyostelium discoideum with altered cyclic GMP metabolism. Journal of General Microbiology 127:339–350
    [Google Scholar]
  27. Scatchard G. 1949; The attractions of proteins for small molecules and ions. Annals of the New York Academy of Sciences 51:660–672
    [Google Scholar]
  28. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. Methods in Cell Physiology 2:397–410
    [Google Scholar]
  29. Wallace J. S., Newell P. C. 1982; Genetic analysis by mitotic recombination in Dictyostelium discoideum of growth and developmental loci on linkage group VII. Journal of General Microbiology 128:953–964
    [Google Scholar]
  30. Wurster B., Schubiger K., Wlck U., Gerisch G. 1977; Cyclic GMP in Dictyostelium discoideum, oscillations and pulses in response to folic acid and cyclic AMP signals. FEBS Letters 76:141–144
    [Google Scholar]
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