1887

Abstract

An extracellular pectinolytic enzyme produced by isolated from the bovine rumen was studied. The enzyme had a pH optimum of 8·0 to 8·5 and was stimulated by Ca and inhibited by EDTA. The products of pectinolysis had an absorption peak at 235 nm and reacted with thiobarbituric acid, indicating a lyase type of action. The enzyme cleaved the substrates terminally from the reducing end; action on poly- and oligogalacturonates resulted in the formation of an unsaturated trigalacturonate. The enzyme was classified as an exopectate lyase (EC 4.2.2.9). A pectinesterase was also produced by but polygalacturonase was not detected.

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/content/journal/micro/10.1099/00221287-128-11-2661
1982-11-01
2021-10-16
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