1887

Abstract

A new method for measuring intracellular pH, employing the intrinsic fluorescent pigments of the plasmodia of the myxomycete , was used to study the role of pH in chemotactic transduction in the plasmodia. The cell became acidified following stimulation with the attractants alanine, glucose, galactose and maltose when their concentrations exceeded the respective thresholds of chemoreception and taxis. The degree of cell acidification paralleled the relaxing tendency in tension generation. A non-metabolizable attractant. 2-deoxyglucose, also acidified the cell. However, the repellent salts NaCl, KCl and CaCl did not change the intracellular pH. Our results suggest that the effects of attractants are mediated by intracellular pH, while the effects of repellents are transduced by ATP as reported previously.

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/content/journal/micro/10.1099/00221287-128-11-2647
1982-11-01
2021-05-13
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References

  1. Ataka M., Tsuchii A., Ueda T., Kurihara K., Kobatake Y. 1978; Comparative studies on the reception of bitter stimuli in the frog, Tetrahymena, slime mold and Nitella. Comparative Biochemistry and Physiology 61A:109–115
    [Google Scholar]
  2. Begg D. A., Rebhum L. I. 1979; pH regulates the polymerization of actin in the sea urchin egg cortex. Journal of Cell Biology 83:241–248
    [Google Scholar]
  3. Camp W. G. 1936; A method of cultivating myxomycete plasmodia. Bulletin of the Torrey Botanical Club 63:205–210
    [Google Scholar]
  4. Carlile M. J. 1970; Nutrition and chemotaxis in the myxomycete Physarumpolycephalum: the effect of carbohydrates on the plasmodium. Journal of General Microbiology 63:221–226
    [Google Scholar]
  5. Chet I., Naveh A., Henis Y. 1977; Chemotaxis of Physarumpolycephalum towards carbohydrates, amino acids and nucleotides. Journal of General Microbiology 102:145–148
    [Google Scholar]
  6. Daniel J. W. 1966; Light-induced synchronous sporulation of a myxomycete - the relation of initial metabolic changes to the establishment of a new cell state. In Cell Synchrony pp. 117–152 Cameron I. L., Padilla G. M. Edited by New York & London: Academic Press;
    [Google Scholar]
  7. Hato M., Ueda T., Kurihara K., Kobatake Y. 1976a; Changes in zeta potential and membrane potential of slime mold Physarum polycephalum in response to chemical stimuli. Biochimica et biophysica acta 426:73–80
    [Google Scholar]
  8. Hato M., Ueda T., Kurihara K., Kobatake Y. 1976b; Phototaxis in the true slime mold Physarum polycephalum. Cell Structure and Function 1:155–164
    [Google Scholar]
  9. Hellewell S. B., Taylor D. L. 1979; The contractile basis of ameboid movement VI.The solation-contraction coupling hypothesis. Journal of Cell Biology 83:633–648
    [Google Scholar]
  10. Hirose T., Ueda T., Kobatake Y. 1980; Changes in ATP concentration triggered by chemoreception in the plasmodia of the myxomycete Physarum polycephalum. Journal of General Microbiology 121:175–180
    [Google Scholar]
  11. Ishida N., Kurihara K., Kobatake Y. 1977; Changes in adhesive properties of the slime mold Physarum polycephalum accompanied with chemoreception. Cytobiologie, 15:269–274
    [Google Scholar]
  12. Ishida N., Kurihara K., Kobatake Y. 1978; Dynamic conformational changes of surface membrane of the true slime mold in response to chemoreception revealed by reactivity with chemical agents. Comparative Biochemistry and Physiology 60:241–246
    [Google Scholar]
  13. Johnson J. J., Epel D., Paul M. 1976; Intracellular pH and activation of sea urchin eggs after fertilization. Nature; London: 262:661–664
    [Google Scholar]
  14. Kincaid R. L., Mansour T. E. 1978; Chemotaxis toward carbohydrates and aminoacids in Physarum polycephalum. Experimental Cell Research 116:377–385
    [Google Scholar]
  15. Kincaid R. L., Mansour T. E. 1979; Cyclic 3´,5´- AMP phosphodiesterase in Physarum polycephalum. I. Chemotaxis toward inhibitors and cyclic nucleotides. Biochimica et biophysica acta 588:332–341
    [Google Scholar]
  16. Knowles D. J. C., Carlile M. J. 1978; The chemotactic response of plasmodia of the myxomycetePhysarum polycephalum to sugars and related compounds. Journal of General Microbiology 108:17–25
    [Google Scholar]
  17. Shen S. S., Steinhardt R. A. 1978; Direct measurement of intracellular pH during metabolic depression of the sea urchin egg. Nature; London: 272:253–254
    [Google Scholar]
  18. Taylor D. L. 1976; Quantitative studies on the polarization optical properties of striated muscle. Journal of Cell Biology 68:497–511
    [Google Scholar]
  19. Tilney L. G., Kiehart D. P., Sardet C., Tilney M. 1978; Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm. Journal of Cell Biology 77:536–550
    [Google Scholar]
  20. Ueda T., Kobatake Y. 1977a; Hydrophobicity of biosurfaces as shown by chemoreceptive thresholds in Tetrahymena, Physarum and Nitella. Journal of Membrane Biology 34:351–368
    [Google Scholar]
  21. Ueda T., Kobatake Y. 1977b; Changes in membrane potential, zeta potential and chemotaxis of Physarum polycephalum in response to n-alcohols, n-aldehydes and n-fatty acids. Cytobiologie 16:16–26
    [Google Scholar]
  22. Ueda T., Kobatake Y. 1979; Spectral analysis of fluorescence of 8-anilino-l-naphthalenesulfonate in chemoreception with a white plasmodium of Physarum polycephalum: evidence for conformational change in the chemoreceptive membrane. Biochimica et biophysica acta 557:199–207
    [Google Scholar]
  23. Ueda T., Terayama K., Kurihara K., Kobatake Y. 1975; Threshold phenomena in chemoreception and taxis in the slime mould Physarum polycephalum. Journal of General Physiology 65:223–234
    [Google Scholar]
  24. Ueda T., Muratsugu M., Kurihara K., Kobatake Y. 1976; Chemotaxis in Physarum polycephalum : effects of chemicals on isometric tension of the plasmodial strand in relation to chemotactic movement. Experimental Cell Research 100:337–344
    [Google Scholar]
  25. Yoshimoto Y., Nakamura S., Kamiya N. 1981; Changes in intracellular Ca2+ and ATP concentrations and intracellular pH of Physarum plasmodium in relation to its contraction-relaxation cycle. In Proceedings of the VIIth Conference of National Institute for Basic Biology on Mechanism of Cell Movement III, Okazaki, Japan 7 pp. 45–47 Kamiyia N., Ishikawa H. Edited by
    [Google Scholar]
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