1887

Abstract

Decarboxylation of ferulate to 4-vinylguaiacol is associated with the production of a phenolic off-flavour in beer. The ability (Pof) of non-brewing strains of to carry out this reaction has been assigned, by tetrad analysis, to a single nuclear gene. This gene () is dominant in heterozygous diploids and segregates independently from a/, and . Therefore, elimination of the Pof phenotype from strains intended for brewing is feasible by either mutation or genetic segregation. Since ferulate was decarboxylated by cell-free supernatants derived from Pof strains, but not by similar fractions from Pof strains, encodes production of a cellular decarboxylase. Strains carrying also decarboxylated coumarate and cinnamate , but with caffeate, 4-hydroxybenzoate, 4-hydroxyphenylacetate, styrylacetate, mandelate or phenylpropionate as substrates, the corresponding decarboxylation products were not detected. may confer resistance to inhibitory effects of naturally occurring cinnamates on yeast growth.

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1982-11-01
2021-05-18
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