SUMMARY: Decarboxylation of ferulate to 4-vinylguaiacol is associated with the production of a phenolic off-flavour in beer. The ability (Pof) of non-brewing strains of to carry out this reaction has been assigned, by tetrad analysis, to a single nuclear gene. This gene () is dominant in heterozygous diploids and segregates independently from α, and Therefore, elimination of the Pof phenotype from strains intended for brewing is feasible by either mutation or genetic segregation. Since ferulate was decarboxylated by cell-free supernatants derived from Pof strains, but not by similar fractions from Pof strains, encodes production of a cellular decarboxylase. Strains carrying also decarboxylated coumarate and cinnamate , but with caffeate, 4-hydroxybenzoate, 4-hydroxyphenylacetate, styrylacetate, mandelate or phenylpropionate as substrates, the corresponding decarboxylation products were not detected. may confer resistance to inhibitory effects of naturally occurring cinnamates on yeast growth.


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