1887

Abstract

The efflux of Ca from the yeast is induced by the influx of mono- or divalent cations. The mechanism of the tight coupling between K influx and Ca efflux was investigated. Two possibilities were examined: (1) Ca efflux is induced only by the carriermediated and energy-dependent K uptake or (2) Ca efflux can be induced by electric coupling to K influx which is driven by its inward electrochemical gradient in the presence of K ionophores. It was found that K influx in the presence of valinomycin or nigericin and in the absence of glucose did not induce Ca efflux. It is suggested, therefore, that the coupling is mediated by antiport carriers which exchange Ca and K.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2611
1982-11-01
2024-12-08
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2611.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2611&mimeType=html&fmt=ahah

References

  1. Armstrong W. MCD., Rothstein A. 1967; Discrimination between alkali metal cations by yeast. II. Cation interactions in transport. Journal of General Physiology 50:967–988
    [Google Scholar]
  2. Boutry M., Foury F., Goffeau A. 1977; Energy-dependent uptake of calcium by the yeastSchizosaccharomyces pombe. Biochimica et biophysica acta 464:602–612
    [Google Scholar]
  3. Conway E. J., Duggan F. 1958; A cation carrier in the yeast cell wall. Biochemical Journal 69:265–274
    [Google Scholar]
  4. Crompton M., Heid I. 1978; The cycling of calcium, sodium and protons across the inner membrane of cardiac mitochondria. European Journal of Biochemistry 91:599–608
    [Google Scholar]
  5. Derks W. J., Borst-Pauwels G. W. 1980; Interaction of monovalent cations with Rb and Na uptake in yeast. Biochimica et biophysica acta 596:381–392
    [Google Scholar]
  6. Eilam Y. 1982a; Effect of monovalent cations on calcium efflux in yeasts. Biochimica et biophysica acta 687:8–16
    [Google Scholar]
  7. Eilam Y. 1982b; Studies on calcium efflux in the yeastSaccharomyces cerevisiae. Microbios in the Press
    [Google Scholar]
  8. Fuhrmann G. F., Rothstein A. 1968; The transport of Zn2+, Co2+ and Ni2+ into yeast cells. Biochimica et biophysica acta 163:325–330
    [Google Scholar]
  9. Harold F. M., Altendorf K. H., Hirata H. 1974; Probing membrane transport mechanisms with ionophores. Annals of the New York Academy of Sciences 235:149–160
    [Google Scholar]
  10. Lichko L. P., Okorokov L. A., Kulaev I. S. 1980; Role of vacuolar ion pool in Saccharomyces carlsbergensis: potassium efflux from vacuoles is coupled with manganese or magnesium influx. Journal of Bacteriology 144:666–671
    [Google Scholar]
  11. Norris P. R., Kelly D. P. 1977; Accumulation of cadmium and cobalt bySaccharomyces cerevisiae. Journal of General Microbiology 99:317–324
    [Google Scholar]
  12. Peña A. 1975; Studies on the mechanism of K+transport in yeast. Archives of Biochemistry and Biophysics 167:397–409
    [Google Scholar]
  13. Rothstein A. 1974; Relationship of cations influxes and effluxes in yeast. Journal of General Physiology 64:608–621
    [Google Scholar]
  14. Ryan J. P., Ryan H. 1972; The role of intracellular pH in the regulation of cation exchanges in yeast. Biochemical Journal 128:139–146
    [Google Scholar]
/content/journal/micro/10.1099/00221287-128-11-2611
Loading
/content/journal/micro/10.1099/00221287-128-11-2611
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error