1887

Abstract

Proline and the solute used to control the osmotic potential of the medium, either sucrose or KCl, accumulated in the hyphae of as the steady state external potential deceased. Proline also accumulated in three other lower fungi. Very little polyol was detected in any of the species analysed. Proline was lost following hypoosmotic shock and accumulated after hyperosmotic shock in . The time taken for growth to resume after shock treatment was variable, but less than 5 h. Potassium was the predominant cation in hyphae grown on sucrose but chloride was insufficient to maintain electroneutrality. The potassium to sodium ratio followed a similar pattern to the radial growth rate with respect to external potential, with maxima at a potential slightly below the highest potential tested. When the osmoticum was sucrose, the calculated internal potential was 20% greater than the observed osmotic potential, but when the osmoticum was KCl, the calculated potential was too high by a factor of more than two, probably indicating error in assumptions concerning the location of ions within the hyphae.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2583
1982-11-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2583.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2583&mimeType=html&fmt=ahah

References

  1. Boonsaeng V., Sullivan P. A., Shepherd M. G. 1976; Mannitol production in fungi during glucose catabolism. Canadian Journal of Microbiology 22:808–816
    [Google Scholar]
  2. Brown L. M., Hellebust J. A. 1978; Sorbitol and proline as intracellular osmotic solutes in the green alga Stichococcus bacillaris. Canadian Journal of Botany 56:676–679
    [Google Scholar]
  3. Christian J. H. B., Hall J. M. 1972; Water relations of Salmonella oranienburg: accumulation of potassium and amino acids during respiration. Journal of General Microbiology 70:497–506
    [Google Scholar]
  4. Chu T. M., Aspinall D., Paleg L. G. 1976; Stress metabolism. VIII. Specific ion effects on proline accumulation in barley. Australian Journal of Plant Physiology 3:503–511
    [Google Scholar]
  5. Cooke R., Kuntz I. D. 1974; The properties of water in biological systems. Annual Review of Biophysics and Bioengineering 3:95–126
    [Google Scholar]
  6. Curtis F. C., Lewis D. H., Cooke R. C. 1980; Distribution of a pentitol in Mucorales. Transactions of the British Mycological Society 74:421–423
    [Google Scholar]
  7. Duniway J. M. 1979; Water relations of water molds. Annual Review of Phytopathology 17:431–460
    [Google Scholar]
  8. Griffin D. M. 1981; Water and microbial stress. Advances in Microbial Ecology 5:91–136
    [Google Scholar]
  9. Liu M. S., Hellebust J. A. 1976; Effects of salinity and osmolarity of the medium on amino acid metabolism in Cyclotella cryptica. Canadian Journal of Botany 54:938–948
    [Google Scholar]
  10. Luard E. J. 1980 Water relations of fungi with particular reference to xerophytic species Ph.D. thesis Australian National University:
    [Google Scholar]
  11. Luard E. J. 1982a; Accumulation of intracellular solutes by two filamentous fungi in response to growth at low steady state osmotic potential. Journal of General Microbiology 128:2563–2574
    [Google Scholar]
  12. Luard E. J. 1982b; Effect of osmotic shock on some intracellular solutes in two filamentous fungi. Journal of General Microbiology 128:2575–2581
    [Google Scholar]
  13. Luard E. J., Griffin D. M. 1981; Effect of water potential on fungal growth and turgor. Transactions of the British Mycological Society 76:33–40
    [Google Scholar]
  14. Measures J. C. 1975; Role of amino acids in osmoregulation of non-halophilic bacteria. Nature; London: 257398–400
    [Google Scholar]
  15. Pfyffer G. E., Rast D. M. 1980; The polyol pattern of some fungi not hitherto investigated for sugar alcohols. Experimental Mycology 4:160–170
    [Google Scholar]
  16. Robinson R. A., Stokes R. H. 1955 Electrolyte Solutions New York: Academic Press;
    [Google Scholar]
  17. Storey R., Wyn Jones R. G. 1977; Quarternary ammonium compounds in plants in relation to salt resistance. Phytochemistry 16:447–453
    [Google Scholar]
  18. Takahashi T., Asai T. 1933; Fermentation products of Mucor. III. Production of glycerol and the effect of the addition of NaHSO3 and Na2CO3. Journal of the Agricultural Chemical Society of Japan 9:443–448
    [Google Scholar]
  19. Te Strake D. 1959; Estuarine distribution and saline tolerance of some Saprolegniaceae. Phyton 12:147–152
    [Google Scholar]
  20. Tresner H. D., Hayes J. A. 1971; Sodium chloride tolerance of terrestrial fungi. Applied Microbiology 22:210–213
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-11-2583
Loading
/content/journal/micro/10.1099/00221287-128-11-2583
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error