1887

Abstract

A comparison was made of kinetics of solute accumulation by NCYC 366 grown anaerobically under conditions that lead to enrichment of the plasma membrane with ergosterol and either oleyl or linoleyl residues. Values for and were identical for accumulation of -asparagine, -glutamine, HPO , Ca and SO , while for accumulation of -glucose, the value differed slightly but not significantly. Values for for accumulation of -lysine, by both the low-and high-affinity systems, decreased when oleyl residues were replaced by linoleyl residues. Under these conditions, values for the high-affinity system decreased while that for the low-affinity system increased. An Arrhenius plot for accumulation of lysine by the high-affinity system revealed a discontinuity when membranes were enriched in linoleyl residues. However, no discontinuity was evident on plots of lysine accumulation when membranes were enriched in oleyl residues. Similar plots for accumulation of -asparagine, which was used as a control, showed that substitution of linoleyl for oleyl residues significantly raised the transition temperature, but had little effect on the activation energy at temperatures below the discontinuity. When palmitoleyl residues were incorporated into the yeast plasma membrane, the value for -lysine accumulation by the high-affinity system was hardly altered, although the value was lowered, as compared with organisms with membranes enriched in oleyl residues. Replacement of oleyl by palmitoleyl residues lowered both the and values for accumulation of -asparagine. A modified statistical method is described for calculating confidence limits for transition points on Arrhenius plots.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-11-2547
1982-11-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/11/mic-128-11-2547.html?itemId=/content/journal/micro/10.1099/00221287-128-11-2547&mimeType=html&fmt=ahah

References

  1. Aiyappa P. S., Lampen J. O. 1976; Membrane associated phospholipoproteins of Bacillus licheniformis. Biochimica et biophysica acta 448:401–410
    [Google Scholar]
  2. Alterthum F., Rose A. H. 1973; Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids. Journal of General Microbiology 77:371–382
    [Google Scholar]
  3. Andreasen A. A., Stier T. J. B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 41:23–36
    [Google Scholar]
  4. Andreasen A. A., Stier T. J. B. 1954; Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43:271–281
    [Google Scholar]
  5. Beepe J. L. 1972; Transport alterations in a phospha- tidylethanolamine-deficient mutant of Bacillus sub- tilis. Journal of Bacteriology 109:939–942
    [Google Scholar]
  6. Bevers E. M., Snoek G. T., Op Den Kamp J. A. F., Van Deenen L. L. M. 1977; Phospholipid requirement of the membrane-bound Mg++ dependent ATPase in Acholeplasma laidlawii. Biochimica et biophysica acta 467:346–356
    [Google Scholar]
  7. Borst-Pauwels G.W.F.H., Jager S. 1969; Inhibition of phosphate and arsenate uptake in yeast by monoiodoacetate, fluoride, 2,4-dinitrophenol and acetate. Biochimica et biophysica acta 172:399–406
    [Google Scholar]
  8. Breton A., Surdin-Kerjan Y. 1977; Sulphate uptake in Saccharomyces cerevisiae: biochemical and genetic study. Journal of Bacteriology 132:224–232
    [Google Scholar]
  9. Cox G. S., Weissbach H., Kaback H. R. 1975; Transport in an Escherichia coli fatty acid auxotroph: a novel case of catabolite repression. Journal of Biological Chemistry 250:4542–4548
    [Google Scholar]
  10. Cunningham C. C., Hager L. P. 1971; Crystalline pyruvate oxidase from Escherichia coli. II. Activation by phospholipids. Journal of Biological Chemistry 246:1575–1582
    [Google Scholar]
  11. Dufour J. P., Goffeau A. 1980; Phospholipid reactivation of the purified plasma membrane ATPase of yeast. Journal of Biological Chemistry 255:10591–10598
    [Google Scholar]
  12. Dunlop P. C., Roon R. J. 1975; l-Asparaginaseof Saccharomyces cerevisiae: an extracellular enzyme. Journal of Bacteriology 122:1017–1024
    [Google Scholar]
  13. Dunlop P. C., Meyer G. M., Ban D., Roon R. J. 1978; Characterization of two forms of asparaginase in Saccharomyces cerevisiae. Journal of Biological Chemistry 253:1297–1304
    [Google Scholar]
  14. Eddy A. A. 1982; Mechanisms of solute transport in selected eukaryotic micro-organisms. Advances in Microbial Physiology 23:1–78
    [Google Scholar]
  15. Folch J., Lees M., Sloane Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry 226:497–509
    [Google Scholar]
  16. Fuhrmann G. F., Rothstein A. 1968; The transport of Zn2+, Co2+ and Ni2+ into yeast cells. Bio- chimica et biophysica acta 163:325–330
    [Google Scholar]
  17. Gennis R. B., Strominger J. L. 1976; Activation °f C55-isoprenoid alcohol phosphokinase from Staphylococcus aureus. I. Activation by phospholipids and fatty acids. Journal of Biological Chemistry 251:1264–1269
    [Google Scholar]
  18. Hinckley A., Mueller E., Rothfield L. 1972; Reassembly of a membrane bound multienzyme system. I. Formation of particles containing phospha- tidylethanolamine, lipopolysaccharide and two glycosyltransferase enzymes. Journal of Biological Chemistry 247:2623–2628
    [Google Scholar]
  19. Hinkley D. V. 1969; Inference about the intersection in two-phase regression. Biometrika 56:495–504
    [Google Scholar]
  20. Hinkley D. V. 1971; Inference in two-phase regression. Journal of the American Statistical Association 66:736–743
    [Google Scholar]
  21. Hofstee B. H. J. 1959; Non-inverted versus inverted plots in enzyme kinetics. Nature; London: 1841296–1298
    [Google Scholar]
  22. Hossack J. A., Rose A. H. 1976; Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols. Journal of Bacteriology 127: 67–75
    [Google Scholar]
  23. Hunter K., Rose A. H. 1972; Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature. Biochimica et biophysica acta 260:639–653
    [Google Scholar]
  24. Inouye M. 1974; A three dimensional molecular assembly model of a lipoprotein from the Escherichia coli outer membrane. Proceedings of the National Academy of Sciences of the United States of America 71:2396–2400
    [Google Scholar]
  25. Keenan M. H. J., Rose A. H. 1979; Plasma-membrane lipid unsaturation can affect the kinetics of solute accumulation by Saccharomyces cerevisiae. FEMS Microbiology tetters 6:133–137
    [Google Scholar]
  26. Kotyk A. 1967; Properties of the sugar carrier in baker’s yeast. Folia microbiologica 12:121–128
    [Google Scholar]
  27. Light R. J., Lennarz W. J., Bloch K. 1962; The metabolism of hydroxystearic acids in yeast. Journal of Biological Chemistry 237:1793–1800
    [Google Scholar]
  28. Neal J. L. 1972; Analysis of Michaelis kinetics for two independent, saturable membrane transport functions. Journal of Theoretical Biology 35:113–118
    [Google Scholar]
  29. Opekarova M., Kotyk A., Horak J. 1975; Isolation and properties of an arginine-binding protein from Saccharomyces cerevisiae. European Journal of Biochemistry 59:373–376
    [Google Scholar]
  30. Peter H. W., Ahlers J. 1975; Phospholipid requirements of ATPase of Escherichia coli. Archives of Biochemistry and Biophysics 170:169–178
    [Google Scholar]
  31. Proudlock J. W., Wheeldon L. W., Jollow D. J., Linnane A. W. 1968; Role of sterols in Saccharomyces cerevisiae. Biochimica et biophysica acta 152:434–437
    [Google Scholar]
  32. Ratcliffe S. J., Hossack J. A., Wheeler G. E., Rose A. H. 1973; Modification of the phospholipid composition of Saccharomyces cerevisiae induced by exogenous ethanolamine. Journal of General Microbiology 76:445–449
    [Google Scholar]
  33. Rose A. H. 1977; Dialling the composition of the yeast plasma membrane. In Alcohol, Industry and Research pp. 179–189 Forsander O., Eriksson K., Oura E., Jounela-Eriksson P. Edited by Helsinki, Finland: Alko;
    [Google Scholar]
  34. Salton M. R. J., Schor M. T. 1974; Release and purification of Micrococcus lysodeikticus ATPase from membranes extracted with n-butanol. Biochimica et biophysica acta 345:74–82
    [Google Scholar]
  35. Sanderman H. 1978; Regulation of membrane enzymes by lipids. Biochimica et biophysica acta 515:209–237
    [Google Scholar]
  36. Scarborough G. A. 1973; Transport in Neurospora. International Review of Cytology 34:103–122
    [Google Scholar]
  37. Sherr S. I. 1969; Phosphatidylcholine deletion from the membrane phospholipids of Neurospora crassa and its effect on lysine uptake. Bacteriological Proceedings120–121
    [Google Scholar]
  38. Skipski V. P., Peterson R. F., Barclay M. 1964; Quantitative analysis of phospholipids by thin-layer chromatography. Biochemical Journal 90:374–384
    [Google Scholar]
  39. Thomas D. S., Hossack J. A., Rose A. H. 1978; Plasma-membrane lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Archives of Microbiology 117:239–245
    [Google Scholar]
  40. Watson K., Rose A. H. 1980; Fatty-acyl composition of the lipids of Saccharomyces cerevisiae grown aerobically or anaerobically in media containing different fatty acids. Journal of General Microbiology 117:225–233
    [Google Scholar]
  41. Wickerham L. J. 1951; Taxonomy of yeasts. I. Techniques of classification. United States Department of Agriculture Technical Bulletin 1029 Washington, D.C: U.S. Department of Agriculture;
    [Google Scholar]
  42. Wiemken A., Dürr M. 1974; Characterization of amino-acid pools in the vacuolar compartment of Saccharomyces cerevisiae. Archives of Microbiology 101:45–58
    [Google Scholar]
/content/journal/micro/10.1099/00221287-128-11-2547
Loading
/content/journal/micro/10.1099/00221287-128-11-2547
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error