SUMMARY: Phages C-2 and J were isolated from sewage. Phage C-2 was filamentous and formed plaques on strains carrying various C plasmids. It also plated on and strains carrying particular C plasmids, but failed to form plaques on lines of K12 strains harbouring most of these plasmids, although in all cases, phage multiplication on the strains was demonstrated. No phage increase occurred in any strain which lacked a C plasmid or contained plasmids of other incompatibility groups. The phage was sensitive to chloroform and, unlike other filamentous bacterial viruses, adsorbed to shafts of conjugative pili. It had a disc-like structure at the end which attached to the pilus. Phage C-2 had a buoyant density of 1.30 g cm and a single-stranded circular DNA genome of 3.0 MDal. Phage J had an hexagonal head with an inter-apical distance of 40 nm and a short non-contractile tail. It was resistant to chloroform and diethyl ether. The phage formed plaques or propagated on strains harbouring some IncC plasmids and all IncJ and IncD plasmids tested. The phage did not form plaques but propagated on and strains carrying these plasmids. It did not plate or propagate on strains harbouring the plasmids. The plaques were very hazy and variable in size. The phage attached sparsely, at a site which appeared to be located at the base of the tail, to sides of conjugative pili.


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