SUMMARY: Synchronous cultures of the cellular slime mould, , were prepared by a selection method which involved low-speed centrifugation of exponential cultures. The method gave a relatively high degree of synchronous growth and cell doubling and a discrete phase of DNA synthesis was discernible. Amoebae which were induced to sporulation at different times during synchronous growth differed in their differentiation responses. Those which were about to divide produced more fruiting bodies and had higher levels of α-mannosidase and -acetylglucosaminidase than cells removed to sporulation conditions at other times during synchronous growth.


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