1887

Abstract

Synchronous cultures of the cellular slime mould, , were prepared by a selection method which involved low-speed centrifugation of exponential cultures. The method gave a relatively high degree of synchronous growth and cell doubling and a discrete phase of DNA synthesis was discernible. Amoebae which were induced to sporulation at different times during synchronous growth differed in their differentiation responses. Those which were about to divide produced more fruiting bodies and had higher levels of α-mannosidase and -acetylglucosaminidase than cells removed to sporulation conditions at other times during synchronous growth.

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/content/journal/micro/10.1099/00221287-128-10-2449
1982-10-01
2021-05-16
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References

  1. Ashworth J. M., Watts D. J. 1970; Metabolism of the cellular slime mould Dictyostelium discoideum grown in axenic culture. Biochemical Journal 119:175–182
    [Google Scholar]
  2. Blumenthal L. K., Zahler S. A. 1962; Index for measurement of synchronization of cell population. Science 134:724
    [Google Scholar]
  3. Chagla A. H., Griffiths A. J. 1978; Synchronous cultures of Acanthamoeba castellanii and their use in the study of encystation. Journal of General Microbiology 108:39–43
    [Google Scholar]
  4. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  5. Katz E. R., Bourguignon L. Y. W. 1974; The cell cycle and its relationship to aggregation in the cellular slime-mould Dictyostelium discoideum. Developmental Biology 36:82–87
    [Google Scholar]
  6. Kissane J. M., Robins E. 1955; The fluorimetric measurement of DNA in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry 233:184–188
    [Google Scholar]
  7. Lloyd D., John L., Edwards C., Chagla A. H. 1975; Synchronous cultures of micro-organisms: large-scale preparation by continuous-flow size selection. Journal of General Microbiology 88:153–158
    [Google Scholar]
  8. Loomis W. F. 1969; Acetylglucosaminidase, an early enzyme in the development of Dictyostelium discoideum. Journal of Bacteriology 97:1149–1154
    [Google Scholar]
  9. Loomis W. F. 1970; Developmental regulation of a- mannosidase in Dictyostelium discoideum. Journal of Bacteriology 103:375–381
    [Google Scholar]
  10. Loomis W. F. 1971; Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Experimental Cell Research 64:484–486
    [Google Scholar]
  11. Loomis W. F. 1975 Dictyostelium discoideum: a Developmental System New York & London: Academic Press;
    [Google Scholar]
  12. Mitchison J. M. 1971 The Biology of the Cell Cycle London: Cambridge University Press;
    [Google Scholar]
  13. Sussman R. R., Sussman M. 1967; Cultivation of Dictyostelium discoideum in axenic medium. Biochemical and Biophysical Research Communications 29:53–55
    [Google Scholar]
  14. Watts D., Ashworth J. M. 1970; Growth of myxamoebae of the cellular slime-mouldDictyostelium discoideumin axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
  15. Zada-Hames I. M., Ashworth J. M. 1978; The cell cycle during the vegetative stage of Dictyostelium discoideum and its response to temperature change. Journal of Cell Science 32:1–20
    [Google Scholar]
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