1887

Abstract

Full-thickness human cadaver skin was maintained on the dorso-lateral thoracic region of hairless mice whose immune rejection mechanism was suppressed using anti-mouse-thymocyte globulin. The bacterial profile of the pregrafted skin did not differ significantly from the normal human microflora. In contrast, the murine skin exhibited quantitative and qualitative differences from the human flora, in particular by the complete absence of , the dominant bacterium on sebum-rich areas of human skin. The normal microbial profile of the human grafts was maintained throughout the experimental period despite the novel environmental milieu. There was little contamination of the grafts from the normal murine flora. It was concluded that the grafted human skin would provide a realistic model for studying the ecology of human cutaneous micro-organisms.

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1982-10-01
2021-10-25
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References

  1. Aly R., Maibach H. I., Shinefield H. R., Mandel A., Strauss W. G. 1974; Bacterial interference among Staphylococcus aureus in man. Journal of Infectious Diseases 129:720–724
    [Google Scholar]
  2. Billingham R. E., Medawar P. B. 1951; The technique of free skin grafting in mammals. Journal of Experimental Biology 28:385–402
    [Google Scholar]
  3. Cove J., Eady E. A. 1982; A selective medium for the isolation of cutaneous propionibacteria. Journal of Applied Bacteriology in the Press
    [Google Scholar]
  4. Cunliffe W. J., Cotterill J. A. 1975 The Acnes: Clinical Features, Pathogenesis and Treatment London: W. B. Saunders;
    [Google Scholar]
  5. Fulton J. E., Mcginley K. J., Leyden J. J., Marples R. R. 1968; Gram negative folliculitis in Acne vulgaris. Archives of Dermatology 98:349–353
    [Google Scholar]
  6. Holland K. T., Roberts C. D., Cunliffe W. J., Williams M. 1974; A technique for sampling microorganisms from the pilosebaceous ducts. Journal of Applied Bacteriology 37:289–296
    [Google Scholar]
  7. Jeljaszewicz R. 1981 editor Staphylococci and staphylococcal infections. Chapter 1. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene I.Abteilung Suppl. 10:3–126
    [Google Scholar]
  8. Kellum R. E. 1966; Isolation of human sebaceous glands. Archives of Dermatology 93:610–612
    [Google Scholar]
  9. Kloos W. E., Schleifer K. H. 1975; Isolation and characterisation of staphylococci from human skin. II. Description of four new species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus ho- minis and Staphylococcus simulans. International Journal of Systematic Bacteriology 25:62–79
    [Google Scholar]
  10. Levins R. 1968 Evolution in Changing Environments Princeton, N.J: Princeton University Press;
    [Google Scholar]
  11. Leyden J. J., Marples R. R. 1973; Ecologic principles and antibiotic therapy in chronic dermatoses. Archives of Dermatology 107:208–211
    [Google Scholar]
  12. Leyden J. J., Mcginley K. J., Mills O. H., Klig-Man A. M. 1975; Age-related changes in the resident bacterial flora of the human face. Journal of Investigative Dermatology 65:379–381
    [Google Scholar]
  13. Marples R. R. 1965; The effect of hydration on the bacterial flora of the skin. In Skin Bacteria and their Role in Infection pp. 33–41 Maibach H. I., Hildick-Smith G. Edited by New York: McGraw-Hill;
    [Google Scholar]
  14. Marples R. R., Mcginley K. J. 1974; Coryne- bacterium acnes and other anaerobic diphtheroids from human skin. Journal of Medical Microbiology 7:349–357
    [Google Scholar]
  15. Noble W. C. 1981 Microbiology of Human Skin London: Lloyd-Luke;
    [Google Scholar]
  16. Puhvel S. M., Reisner R. M., Amirian D. A. 1975; Quantification of bacteria in isolated pilose- baceous follicles in normal skin. Journal of Investigative Dermatology 65:525–531
    [Google Scholar]
  17. Shaw C. M., Smith J. A., Mcbride M. E., Duncan W. C. 1970; An evaluation of techniques for sampling skin flora. Journal of Investigative Dermatology 54:160–163
    [Google Scholar]
  18. Somerville D. A., Noble W. C. 1973; Microcolony size of microbes on human skin. Journal of Medical Microbiology 6:323–328
    [Google Scholar]
  19. Webster G. F., Ruggieri M. R., Mcginley K. J. 1981; Correlation of Propionibacterium acnes populations with the presence of triglycerides on nonhuman skin. Applied and Environmental Microbiology 41:1269–1270
    [Google Scholar]
  20. Williamson P., Kligman A. M. 1965; A new method for the quantitative investigation of cutaneous bacteria. Journal of Investigative Dermatology 45:498–503
    [Google Scholar]
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