1887

Abstract

A newly devised method to obtain diffuse growth of A3(2) in liquid minimal medium was used to study glucose repression. Although diauxic growth was not obtained, glucose repression of uptake of C-labelled carbon sources was demonstrated. Active, arabinose-induced, arabinose transport was repressed at the level of transcription by glucose. Of two glycerol-inducible glycerol transport systems, one was glucose-inhibited but not repressed (and operated by facilitated diffusion), whilst the other (an active transport system) was glucose-repressed. Active transport systems for galactose and fructose which did not require induction by their respective sugars were both inhibited by glucose. Galactose- and fructose-metabolizing enzymes were inducible by the respective sugars, but only in the absence of glucose. This was because glucose both inhibited galactose and fructose transport and repressed the metabolic enzymes concerned. Constitutive active glucose uptake was also demonstrated in arabinose-grown cells. Mutants that grew on arabinose or glycerol in the presence of 2-deoxy-glucose were glucose-derepressed for both soluble carbon source utilization and extracellular agarose. Three glucose-derepressed mutants were studied in detail. One of these could not utilize glucose (and probably lacks glucose kinase), whilst the other two could utilize glucose to differing degrees.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-10-2417
1982-10-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/10/mic-128-10-2417.html?itemId=/content/journal/micro/10.1099/00221287-128-10-2417&mimeType=html&fmt=ahah

References

  1. Chater K. F. 1974; Rifampicin-resistant mutants of Slreptomycescoelicolor A3(2). Journal of General Microbiology 80:277–290
    [Google Scholar]
  2. Cochrane V. W. 1961; Physiology of Actino-mycetes. Annual Review of Microbiology 15:1–26
    [Google Scholar]
  3. Heredia C. F., Sols A. 1964; Metabolic studies with 2-deoxyhexoses. II. Resistance to 2-deoxyglucose in a yeast mutant. Biochimica et biophysica acta 86:224–228
    [Google Scholar]
  4. Hodgson D. A. 1980 Carbohydrate utilization in Streptomyces coelicolor A3(2) Ph.D. thesis, University of East Anglia, Norwich, U.K.
    [Google Scholar]
  5. Hodgson D. A., Chater K. F. 1981; A chromosomal locus controlling extracellular agarase production by Streptomyces coelicolor and its inactivation by chromosomal integration of plasmid SCP1. Journal of General Microbiology 124:339–348
    [Google Scholar]
  6. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  7. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor A3(2) genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  8. Kutzner H. J., Waksman S. A. 1959; Streptomyces coelicolor Muller and Streptomyces violaceo-ruber Waksman and Curtis, two distinctly different organisms. Journal of Bacteriology 78:528–538
    [Google Scholar]
  9. Lin E. C. C. 1976; Glycerol dissimilation and its regulation in bacteria. Annual Review of Microbiology 30:535–578
    [Google Scholar]
  10. Lobo Z., Maitra P. K. 1977; Resistance to 2-deoxyglucose in yeast: a direct selection of mutants lacking glucose phosphorylating enzymes. Molecular and General Genetics 157:297–300
    [Google Scholar]
  11. Magasanik B. 1970; Glucose effects: inducer exclusion and repression. In The Lactose Operon pp. 189–219 Edited by Beckwith J. R., Zipser D. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Martin J. F., Demain A. L. 1980; Control of antibiotic biosynthesis. Microbiological Reviews 44:230–251
    [Google Scholar]
  13. Neujahr H. Y., Ewaldsson B. 1964; Counting of weak β-emitters in bacterial cells by means of the liquid scintillation method. Analytical Biochemistry 8:487–494
    [Google Scholar]
  14. Sabater B., Asensio C. 1973; Transport of hexoses in Streptomyces coelicolor. European Journal of Biochemistry 39:201–205
    [Google Scholar]
  15. Sabater B., Sebastian J., Asensio C. 1972; Identification and properties of an inducible and highly specific fructokinase from Streptomyces violaceoruber. Biochimica et biophysica acta 284:414–420
    [Google Scholar]
  16. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of UF to IF strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
  17. Wheelis M. H., Wells B. 1974; Uniform growth of Streptomyces coelicolor in liquid medium. In John Innes Annual Report, 1974 p. 77 Norwich: John Innes Institute;
    [Google Scholar]
  18. Wright L. F., Hopwood D. A. 1976a; Identification of the antibiotic determined by SCP1 plasmid of Streptomyces coelicolor A3(2). Journal of General Microbiology 95:96–106
    [Google Scholar]
  19. Wright L. F., Hopwood D. A. 1976b; Actinorhodin is a chromosomally determined antibiotic in Streptomyces coelicolor A3(2). Journal of General Microbiology 96:289–297
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-10-2417
Loading
/content/journal/micro/10.1099/00221287-128-10-2417
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error