1887

Abstract

The growth rate of cells in a chemically defined medium was inversely proportional to the concentration of Mn between 15 and 300 mu;. In the presence of >100 mu;-Mn, cells grew with doubling times of >60 min. Cessation of exponential growth due to a high concentration of Mn was most pronounced in cultures low in sulphur or carbon. This was due to the toxic effect of high Mn concentrations since dilution of cultures to a final Mn concentration of 15 mu; or less restored the growth rate to maximum. Sporulation depended upon the nature of the growth-limiting nutrient. The manganese effect on sporulation of sulphur-limited cells depended upon the concentration of glucose and the aeration rate in a qualitatively similar manner. The highest spore yield at optimal aeration rates was obtained when the initial Mn concentration in the medium was 10–30 μ. Although the growth rate of bacteria in low-sulphate media was determined by the manganese concentration, its effect on sporulation frequency was independent of the growth rate.

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1982-10-01
2022-01-22
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