Characterization of Cytochromes in Lithotrophically and Organotrophically Grown Cells of A2 Free

Abstract

The dominant cytochrome in thiosulphate-grown A2 was found to be of the -type with a reduced α-band at 548 nm ( ). This component did not constitute an integral part of the membrane carrier system. It did, however, appear to be part of a large complex not tightly bound to membranes. Reconstitution experiments showed that cytochromes of the membrane ‘’ system could be reduced by the component and . The reduction of membrane cytochromes of either lithotrophic or organotrophic origin by thiosulphate electrons was achieved, but it required the presence of a soluble fraction containing cytochrome . Evidence tending to rule out a reductive cleavage as the first step of the thiosulphate oxidation pathway in A2 was obtained by following the reduction of partially purified particles under various conditions.

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1982-10-01
2024-03-29
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