1887

Abstract

produces a phase-specific cationic mucopolysaccharide composed primarily of galactosamine (galactosaminoglycan mucopolysaccharide; GAG-MP), which becomes part of the cell wall and is later secreted into the medium. Its appearance coincides with the onset of the restricted phase of growth, and causes efflux of small molecular weight metabolites when incubated with conidial cells. This activity may be a product of electrostatic interactions of the GAG-MP with the conidial plasma membrane. The activity is blocked by acetylation of the primary amines on the molecule, digestion by enzymes that hydrolyse carbohydrate linkages and the inclusion of NaCl (1 ) in the GAG-MP/conidia reaction mixture. The physiological activity of the GAG-MP mimics that of known depolarizing agents. A model is proposed in which GAG-MP depolarizes the plasma membrane at the onset of the restricted phase of growth, stimulating a cell surface enzyme to produce endogenous cyclic AMP which in turn switches on the enzymic and genetic machinery necessary for that phase of growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-10-2333
1982-10-01
2022-01-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/10/mic-128-10-2333.html?itemId=/content/journal/micro/10.1099/00221287-128-10-2333&mimeType=html&fmt=ahah

References

  1. Harold F. M. 1962; Binding of inorganic polyphosphate to the cell wall of Neurospora crassa. Biochimica et biophysica acta 57:59–66
    [Google Scholar]
  2. Mahadevan P. R., Tatum E. L. 1965; Relationship of the major constituents of the Neurospora cell wall to wild type and colonial morphology. Journal of Bacteriology 90:1073–1081
    [Google Scholar]
  3. Marmur J. 1963; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Methods in Enzymology 6:726–738
    [Google Scholar]
  4. Pall M. L. 1977; Cyclic AMP and the plasma membrane potential in Neurospora crassa. Journal of Biological Chemistry 252:7746–7150
    [Google Scholar]
  5. Quinton P. M., Philpott C. W. 1973; A role for anionic sites in epithelial architecture.Effects of cationic polymers on cell membrane structure. Journal of Cell Biology 56:787–796
    [Google Scholar]
  6. Reissig J. L. 1974; Decoding of regulatory signals at the microbial surface. Current Topics in Microbiology and Immunology 67:43–96
    [Google Scholar]
  7. Reissig J. L., Glasgow J. W. 1971; Mucopolysaccharide which regulates growth in Neurospora. Journal of Bacteriology 106:882–889
    [Google Scholar]
  8. Schmit J. L., Edson C. M., Brody S. 1975; Changes in glucosamine and galactosamine levels during conidial germination in Neurospora crassa. Journal of Bacteriology 122:1062–1070
    [Google Scholar]
  9. Scott W. A., Tatum E. L. 1970; Glucose-6-phosphate dehydrogenase and Neurospora morphology. Proceedings of the National Academy of Sciences of the United States of America 66:515–522
    [Google Scholar]
  10. Slayman C. L. 1965; Electrical properties of Neurospora crassa: effects of external cations on the intracellular potential. Journal of General Physiology 49:69–92
    [Google Scholar]
  11. Terenzi H. F., Flawia M. M., Tellez-Inon M. T., Torrez H. N. 1976; Control of Neurospora crassa morphology by cyclic 3´,5´-monophosphate. Journal of Bacteriology 126:91–99
    [Google Scholar]
  12. Trevillyan J. M., Pall M. L. 1979; Control of cyclic AMP 3´,5´-monophosphate levels by depolarizing agents in fungi. Journal of Bacteriology 138:397–403
    [Google Scholar]
  13. Trevithick J. R., Metzenberg R. L., Costello D. F. 1966; Genetic alterations of pore size and other properties of the Neurospora cell wall. Journal of Bacteriology 92:1016–1020
    [Google Scholar]
  14. Vogel H. J. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 97:435–446
    [Google Scholar]
  15. Wagner W. D. 1979; A more sensitive assay discriminating galactosamine and glucosamine in mixtures. Analytical Biochemistry 94:394–396
    [Google Scholar]
  16. Wheat R. W. 1966; Analysis of hexosamines in bacterial polysaccharide by chromatographic procedures. Methods in Enzymology 8:60–78
    [Google Scholar]
  17. Yphantix D. A., Dainko J. L., Schlenk F. 1967; Effect of some proteins on the yeast membrane. Journal of Bacteriology 94:1509–1515
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-10-2333
Loading
/content/journal/micro/10.1099/00221287-128-10-2333
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error