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Volume 128, Issue 10

Enzymes of -Acetylglucosamine Metabolism during Germ-tube Formation in Free

Abstract

The enzymes of -acetyl--glucosamine (GlcNAc) metabolism, GlcNAc-6-phosphate deacetylase and GlcN-6-phosphate deaminase were found to be inducible in . The pattern of induction for these enzymes was the same under conditions of germ-tube formation (37 °C) and where yeast cells metabolized GlcNAc with no change in morphology (28 °C); this indicates that these enzymes are not control points in the dimorphic development of . During induction there was a 40- and 25-fold increase in specific activity for the deacetylase and the deaminase, respectively, and the maximum specific activity corresponded to the time when all the GlcNAc had been metabolized. The presence of lomofungin (an inhibitor of transcription) or trichodermin (an inhibitor of translation) in cell suspensions of containing GlcNAc prevented the increase in specific activity of these enzymes. 2-Deoxyglucose inhibited germ-tube formation, partially inhibited the induction of the deacetylase (43%) and the deaminase (60%), but did not affect the growth of on either Glc or GlcNAc. GlcN-6-phosphate was a competitive inhibitor of the deacetylase with a of 1·45 m while the other product of the reaction, acetate, did not inhibit the enzyme. The value for GlcN-6-phosphate on GlcN-6-phosphate deaminase was 0·24 m. Incubation of starved yeast cells with GlcNAc produced a four-fold increase in the specific activity of UDP-GlcNAc-pyrophosphorylase at either 28 °C or 37 °C.

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© Society for General Microbiology 1982
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1982-10-01
2024-04-19
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References

  1. Braun P. C., Calderone R. R. 1978; Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms. Journal of Bacteriology 133:1472–1477
     [Google Scholar]
  2. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans. Journal of General Microbiology 77:417–426
     [Google Scholar]
  3. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans. Journal of General Microbiology 51:367–376
     [Google Scholar]
  4. Chiew Y. Y., Sullivan P. A., Shepherd M. G. 1980a; Chitin synthesis during germ-tube formation in Candida albicans. Proceedings of the University of Otago Medical School 58:31–33
     [Google Scholar]
  5. Chiew Y. Y., Shepherd M. G., Sullivan P. A. 1980b; Regulation of chitin synthesis during germtube formation in Candida albicans. Archives of Microbiology 125:97–104
     [Google Scholar]
  6. Dixon M., Webb E. C. 1979 In Enzymes p. 113 Dixon M., Webb E. C. Edited by London: Longman;
     [Google Scholar]
  7. Eggstein M., Kreutz F. H. 1967 In Techniques in Protein Chemistry p. 340 Bailey J. L. Edited by London: Elsevier;
     [Google Scholar]
  8. Eisenthal R., Cornish-Bowden A. 1974; The direct linear plot. Biochemical Journal 139:715–720
     [Google Scholar]
  9. Gopal P. K., Sullivan P. A., Shepherd M. G. 1981; A rapid and sensitive assay for N-acetyl glucosamine-6-phosphate deacetylase. Analytical Biochemistry 115:30–33
     [Google Scholar]
  10. Gopal P. K., Sullivan P. A., Shepherd M. G. 1982; A microassay for UDP-jV-acetylglucosaminepyrophosphorylase. Canadian Journal Biochemistry (in the Press)
    [Google Scholar]
  11. Lampen J. O., Kuo S. C., Cano F. R. 1973; Control of synthesis and excretion of exoenzymes. In Yeast, Mould and Plant Photoplast pp. 143–157 Villanueva J. R., Garcia-Acha I., Gascon S., Uruburu F. Edited by London, New York: Academic Press;
     [Google Scholar]
  12. Mattia E., Cassone A. 1979; Inducibility of germ- tube formation in Candida albicans at different phases of yeast growth. Journal of General Microbiology 113:439–442
     [Google Scholar]
  13. Odds F. C. 1979 In Candida and Candidosis p. 35 Leicester: Leicester University Press;
     [Google Scholar]
  14. Randerath K., Randerath E. 1967; Thin layer-separation methods for nucleic acid derivatives. Methods in Enzymology 12:323–347
     [Google Scholar]
  15. Scherwitz C., Martin R., Veberberg H. 1978; Ultrastructural investigations of the formation of Candida albicans germ tubes and septa. Sabouraudia 16:115–124
     [Google Scholar]
  16. Shepherd M. G., Sullivan P. A. 1976; The production and growth characteristics of yeast and mycelial forms of Candida albicans in continuous culture. Journal of General Microbiology 93:361–370
     [Google Scholar]
  17. Shepherd M. G., Chiew Y. Y., Ram S. P., Sullivan P. A. 1980a; Germ-tube induction in Candida albicans. Canadian Journal of Microbiology 26:21–26
     [Google Scholar]
  18. Shepherd M. G., Ghazali H. M., Sullivan P. A. 1980b; N-acetylglucosamine kinase and germ-tube formation in Candida albicans. Experimental Mycology 4:147–159
     [Google Scholar]
  19. Simonetti N., Strippoli V., Cassone A. 1974; Yeast-mycelial conversion induced by N-acetyl-d-glucosamine in Candida albicans. Nature, London 250:344–346
     [Google Scholar]
  20. Singh B. R., Datta A. 1979a; Induction of N-acetyl glucosamine-catabolic pathway in spheroplasts of Candida albicans. Biochemical Journal 178:427–431
     [Google Scholar]
  21. Singh B. R., Datta A. 1979b; Regulation of glucosamine-6-phosphate deaminase synthesis in yeast. Biochimica et biophysica acta 583:28–35
     [Google Scholar]
  22. White R. J., Pasternak C. K. 1975; N-acetyl glucosamine-6-phosphate deacetylase and glucosamine-6-phosphate deaminase from Escherichia coli. Methods in Enzymology 41:497–502
     [Google Scholar]
  23. Yamamoto K., Kawai H., Moriguchi M., Tochikura T. 1976; Purification and characterisation of yeast UDP-N-acetylclyglucosamine pyrophosphorylase. Agricultural and Biological Chemistry 40:2275–2281
     [Google Scholar]
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Enzymes of N-Acetylglucosamine Metabolism during Germ-tube Formation in Candida albicans
Microbiology 128, 2319 (1982); https://doi.org/10.1099/00221287-128-10-2319
/content/journal/micro/10.1099/00221287-128-10-2319
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