%0 Journal Article %A Attwood, Margaret M. %A Van Dijken, J. P %T Characteristics of Fructose-1,6-bisphosphatase from the Methanol-utilizing Yeast Hansenula polymorpha %D 1982 %J Microbiology, %V 128 %N 10 %P 2313-2317 %@ 1465-2080 %R https://doi.org/10.1099/00221287-128-10-2313 %I Microbiology Society, %X Fructose-1,6-bisphosphatase (EC 3.1.3.11) has been partially purified from methanol- and ethanol-grown Hansenula polymorpha. The enzyme was specific for fructose 1,6-bisphosphate as a substrate and did not react with sedoheptulose 1,7-bisphosphate. The enzyme purified from ethanol-grown cells resembled that from methanol-grown cells in all properties investigated, namely, temperature stability, substrate affinity, pH-dependent inhibition by AMP, and pH-dependent stimulation of activity by EDTA. It is concluded that, although the metabolic role of fructose-1,6-bisphosphatase during growth on methanol and ethanol is completely different, growth on these substrates probably involves the same enzyme. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-10-2313