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SUMMARY: Synthesis of anabolic carbamoyltransferase (EC 2.1.3.3.) of Pseudomonas putida A90 was repressed two- to five-fold when arginine was added to cultures growing at the expense of a readily utilizable substrate, such as asparagine; synthesis was repressed 16- to 17-fold when arginine was the sole carbon or carbon and nitrogen source. The most likely explanation is that asparagine exerted some form of catabolite repression on arginine transport. Bacteria grown in the presence of asparagine plus arginine transported arginine at only 13 % of the rate of bacteria grown at the expense of arginine alone.