%0 Journal Article %A Wiegel, J. %A Quandt, L. %T Determination of the Gram Type Using the Reaction Between Polymyxin B and Lipopolysaccharides of the Outer Cell Wall of Whole Bacteria %D 1982 %J Microbiology, %V 128 %N 10 %P 2261-2270 %@ 1465-2080 %R https://doi.org/10.1099/00221287-128-10-2261 %I Microbiology Society, %X The polymyxin B-dependent formation of protrusions (blebs) of lipopolysaccharides on the cell wall of whole bacteria is suggested as a specific reaction for the determination of the Gram type of newly isolated bacteria. A total of 35 different bacterial species from 27 genera were studied as well as the influence of various test parameters. The reaction was specific: all 13 species of the positive Gram type gave a negative reaction, but all bacteria of the negative Gram type gave a distinct positive reaction. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-10-2261