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Abstract

The polymyxin B-dependent formation of protrusions (blebs) of lipopolysaccharides on the cell wall of whole bacteria is suggested as a specific reaction for the determination of the Gram type of newly isolated bacteria. A total of 35 different bacterial species from 27 genera were studied as well as the influence of various test parameters. The reaction was specific: all 13 species of the positive Gram type gave a negative reaction, but all bacteria of the negative Gram type gave a distinct positive reaction.

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/content/journal/micro/10.1099/00221287-128-10-2261
1982-10-01
2025-03-20
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