1887

Abstract

K99 antigen production by enterotoxigenic strains of bovine origin was investigated by slide agglutination and attachment to intestinal villi. Work with two strains (B41 and B44) showed that on minimal medium M2, K99 antigen was not repressed by a high concentration of glucose (2%, w/v). Growth on synthetic or complex medium did not affect K99 antigen detection, which was independent of capsular antigens, and its synthesis was not repressed by Casamino acids or glucose. A survey of 12 strains revealed two groups: in one group K99 antigen production was constitutive on basal medium without glucose, and in the second group K99 antigen was produced only in the presence of glucose. Immunoelectrophoresis patterns, and the results of slide agglutination and attachment tests, were dependent upon K99 type, whereas haemagglutination patterns were not.

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1982-10-01
2024-04-24
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References

  1. Contrepois M., Girardeau J. P., Dubourguier H. C., Gouet PH., Levieux D. 1978; Specific protection by colostrum from cow vaccinated with K.99 antigen in newborn calves experimentally infected with E. coli Ent+ K99+. Annales de recherches veterinaires 9:385–388
    [Google Scholar]
  2. Contrepois M., Dubourguier H. C., Girardeau J. P., Gouet PH. 1979; Méthodes de diagnostic des infections à Escherichia coli entéropathogenes chez le veau et données épidémiologiques. Groupements techniques vétérinaires No. 80-4B bis- 187:25–36
    [Google Scholar]
  3. Dean A. G., Ching Y. C., Williams R. G., Harden L. B. 1972; Test of Escherichia coli enterotoxin using infant mice: application in a study of diarrhoea in children in Honolulu. Journal of Infectious Diseases 125:407–411
    [Google Scholar]
  4. Dubourguier H. C., Gouet PH., Contrepois M., Girardeau J. P. 1978; Diarrhée du nouveau-né: propriétés et mécanismes d’action des Escherichia coli enteropathogຘnes chez le veau et le porcelet. Annales de recherches vétérinaires 9:129–152
    [Google Scholar]
  5. Evans D. J., Evans D. G., Dupont H. L. 1979; Haemagglutination patterns of enterotoxigenic and enteropathogenic Escherichia coli determined with human, bovine, chicken and guinea pig erythrocytes in the presence and absence of mannose. Infection and Immunity 23:336–346
    [Google Scholar]
  6. Girardeau J. P. 1980; A new in vitro technique for attachment to intestinal villi using enteropathogenic Escherichia coli. . Annales de microbiologie 131B:31–37
    [Google Scholar]
  7. Girardeau J. P., Dubourguier H. C., Gouet PH. 1982; Inhibition of K99 synthesis by L-alanine in enterotoxigenic Escherichia coli. . Journal of General Microbiology 128:463–470
    [Google Scholar]
  8. Guinée P. A. M., Jansen W. H., Agterberg C. 1976; Detection of the K99 antigen by means of agglutination and immunoelectrophoresis in Escherichia coli isolates from calves and its correlation with enterotoxigenicity. Infection and Immunity 13:1369–1377
    [Google Scholar]
  9. Guinée P. A. M., Weldkamp J., Jansen W. H. 1977; Improved minca medium for the detection of K99 antigen in calf enterotoxigenic strains of E. coli. . Infection and Immunity 15:676–678
    [Google Scholar]
  10. De Graaf F. K., Wientjes F. B., Klaasen-Boor P. 1980a; Production of K99 antigen by enterotoxigenic Escherichia coli strains of antigen groups 08, 09, 020 and O101 grown at different conditions. Infection and Immunity 27:216–221
    [Google Scholar]
  11. Degraaf F. K., Klaasen-Boor P., Van Hees J. E. 1980b; Biosynthesis of the K99 surface antigen is repressed by alanine. Infection and Immunity 30:125–128
    [Google Scholar]
  12. Isaacson R. E. 1980; Factors affecting expression of the Escherichia coli pilus K99. Infection and Immunity 28:190–193
    [Google Scholar]
  13. Meyers L. L., Guinée P. A. M. 1976; Occurrence and characteristics of enterotoxigenic Escherichia coli isolated from calves with diarrhea. Infection and Immunity 13:1117–1119
    [Google Scholar]
  14. Moon H. W., Whipp S. C., Skartvedt S. M. 1976; Etiologic diagnosis of diarrheal diseases of calves. Incidence and methods for detecting entero- toxin and K99 antigen production by Escherichia coli. . Annals of Veterinary Research 37:1025–1029
    [Google Scholar]
  15. Morin M., Lariviere S., Lallier R. 1976; Pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea. Canadian Journal of Comparative Medicine 40:228–240
    [Google Scholar]
  16. Morris J. A., Thorns C. J., Sojka W. J. 1980; Evidence for two adhesive antigens on the K99 reference strain Escherichia coli B41. Journal of General Microbiology 118:107–113
    [Google Scholar]
  17. Ørskov I., Ørskov F., Smith H. W., Sojka W. J. 1975; The establishment of K99, a thermolabile, transmissible Escherichia coli K antigen, previously called “KCo”, possessed by calf and lamb enteropathogenic strains. Acta pathologica et microbiologica scandinavica 83:31–36
    [Google Scholar]
  18. Raibaud P., Dickinson A. B., Sacquet E., Char-Lier H., Mocquot G. 1966; La microflore du tube digestif du rat. Techniques d’etude et milieux de culture proposes. Annales de I’Institut Pasteur 110:568–590
    [Google Scholar]
  19. Renault L., Contrepois M., Dubourguier H. C., Gouet PH., Bordas C., Le Bourhis E. 1980; Interest of slide agglutination test K(A) antigen for the detection of enteropathogenic strains of Escherichia coli in diarrhoeic calves. 2éme Symposium International des Vétérinaires de Laboratoires de Diagnostic, Lucerne, 26-27 June 1980, pp. 420–423
    [Google Scholar]
  20. Scheidegger J. J. 1955; Une micro methode d’im- muno-électrophorèse. International Archives of Allergy 7:103–110
    [Google Scholar]
  21. Smith H. W., Lingood M. A. 1972; Further observation on Escherichia coli enterotoxins with particular regard to those produced by atypical piglet strains and by calf and lamb strains, the transmissible nature of these enterotoxins and of a K antigen possessed by calf and lamb strains. Journal of Medical Microbiology 5:243–250
    [Google Scholar]
  22. Sojka W. J. 1965; E coli in domestic animals and poultry. Review, Commonwealth Agricultural Bureaux No. 7 205–214
    [Google Scholar]
  23. Sojka W. J. 1971; Enteric diseases in new-born piglets, calves and lambs due to Escherichia coli infection. Veterinary Bulletin 41:509–522
    [Google Scholar]
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