1887

Abstract

The position of a gene () that is essential for growth of with fumarate or nitrate as electron acceptor was located within an 11·5 kb dIII fragment of bacterial DNA by deletion analysis with transducing phages () and by sub-cloning restriction fragments into multicopy plasmids. The functional gene was isolated in a 1·65 kb HI-dIII fragment of a hybrid plasmid pGS24. The gene product wasidentified as a protein of 31000, by post-infection labelling and by the ‘maxicell’ method. Organisms containing the multicopy plasmid (pGS24) overproduced fumarate reductase to the same extent as cultures containing a comparable fumarate reductase plasmid (pNU3 1) during anaerobic growth; the plasmid also overcame the repression of fumarate reductase synthesis that is normally observed during aerobic growth. Similar effectson nitrate reductase synthesis were also observed. The results support the view that the gene product functions as a positive regulator or a specific sigma factor for expression of anaerobic energy-generating systems.

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1982-10-01
2022-01-20
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