1887

Abstract

Histidine-grown cells of can accumulate more than 100 m free ammonia in the medium. Growth and respiration are unaffected by these concentrations of ammonia. Measurements of internal and external ammonia concentrations for cells grown on histidine or NHCl as nitrogen sources suggest rapid equilibration of ammonia across the cell membrane under conditions where actively accumulated solutes are retained. Implications of these results for movement of ammonia out of N-fixing bacteroids are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-1-29
1982-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/1/mic-128-1-29.html?itemId=/content/journal/micro/10.1099/00221287-128-1-29&mimeType=html&fmt=ahah

References

  1. Bellion E., Khan M. Y. A., Romano M. J. 1980; Transport of methylamine by Pseudomonas sp. MA. Journal of Bacteriology 142:786–790
    [Google Scholar]
  2. Bergersen F. J. 1965; Ammonia-an early stable product of nitrogen fixation by soybean root nodules. Australian Journal of Biological Sciences 18:1–9
    [Google Scholar]
  3. Bergersen F. J., Turner G. L. 1967; Nitrogen fixation by the bacteroid fraction of breis of soybean root nodules. Biochimica et biophysica acta 141:507–515
    [Google Scholar]
  4. Bergersen F. J., Turner G. L. 1978; Activity of nitrogenase and glutamine synthetase in relation to availability of oxygen in continuous cultures of a strain of cowpea Rhizobium sp. supplied with excess ammonium. Biochimica et biophysica acta 538:406–416
    [Google Scholar]
  5. Boland M. J., Farnden K. J. F., Robertson J. G. 1980; Ammonia assimilation in nitrogenfixing legume nodules. In Nitrogen Fixation, vol. 2: Symbiotic Associations and Cyanobacteria pp. 33–52 Edited by Newton W. E., Orme-Johnson W. H. Baltimore: University Park Press;
    [Google Scholar]
  6. Breiman A., Barash I. 1980; Methylamine and ammonia transport in Stemphylium botryosum. . Journal of General Microbiology 116:201–206
    [Google Scholar]
  7. Brown C. M., Dilworth M. J. 1975; Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86:39–48
    [Google Scholar]
  8. Cook R. J., Anthony C. 1978; The ammonia and methylamine active transport system of Aspergillus nidulans. . Journal of General Microbiology 109:265–274
    [Google Scholar]
  9. Dilworth M. J., Kidby D. K. 1967; Localisation of iron and leghaemoglobin in the legume nodule by electron microscope autoradiography. Experimental Cell Research 49:148–159
    [Google Scholar]
  10. Dilworth M. J., Subramanian D., Munson T. O., Burris R. H. 1965; The adenosine triphosphate requirement for nitrogen fixation in cell-free extracts of Clostridium pasteurianum. . Biochimica et biophysica acta 99:486–503
    [Google Scholar]
  11. Fawcett J. K., Scott J. E. 1960; A rapid and precise method for the determination of urea. Journal of Clinical Pathology 13:156–159
    [Google Scholar]
  12. Glenn A. R., Dilworth M. J. 1981; The uptake and hydrolysis of disaccharides by fast- and slow- growing species of Rhizobium. . Archives of Microbiology 129:233–239
    [Google Scholar]
  13. Glenn A. R., Poole P. S., Hudman J. F. 1980; Succinate uptake by free-living and bacteroid forms of Rhizobium leguminosarum. . Journal of General Microbiology 119:267–271
    [Google Scholar]
  14. Hackett S. L., Skye G. E., Burton C., Cervone F. 1970; Characterization of an ammonium transport system in filamentous fungi with methyl-ammonium-14C as the substrate. Journal of Biological Chemistry 245:4241–4250
    [Google Scholar]
  15. Hudman J. F., Glenn A. R. 1980; Glucose uptake by free-living and bacteroid forms of Rhizobium leguminosarum. . Archives of Microbiology 128:72–77
    [Google Scholar]
  16. Kennedy I. R. 1966a; Primary products of symbiotic nitrogen fixation. I. Short-term exposures of serradella nodules to l5N2. Biochimica et biophysica acta 130:284–294
    [Google Scholar]
  17. Kennedy I. R. 1966b; Primary products of symbiotic nitrogen fixation. II. Pulse labelling of serradella nodules with l5N2. Biochimica et biophysica acta 130:295–303
    [Google Scholar]
  18. Kurz W. G. W., Rokosh D. A., Larue T. A. 1975; Enzymes of ammonia assimilation in Rhizobium leguminosarum bacteroids. Canadian Journal of Microbiology 21:1009–1012
    [Google Scholar]
  19. Laane C., Krone W., Haaker H., Veeger C. 1980; Short-term effect of ammonium chloride on nitrogen fixation by Azotobacter vinelandii and by bacteroids of Rhizobium leguminosarum. . European Journal of Biochemistry 103:39–46
    [Google Scholar]
  20. Mcparland R. H., Guevara J. G., Becker R. R., Evans H. J. 1976; The purification and properties of the glutamine synthetase from the cytosol of soybean root nodules. Biochemical Journal 153:597–606
    [Google Scholar]
  21. O’Gara F., Shanmugam K. T. 1976; Regulation of nitrogen fixation by rhizobia. Export of fixed N2 as NH4+. Biochimica et biophysica acta 437:313–321
    [Google Scholar]
  22. Ray W. J. 1967; Photochemical oxidation. Methods in Enzymology 11:490–497
    [Google Scholar]
  23. Rechler M. M., Tabor H. 1971; Histidine ammonia lyase (Pseudomonas). Methods in Enzymology 2:63–69
    [Google Scholar]
  24. Robertson J. G., Farnden K. J. F., Banks J. 1975a; Induction of glutamine synthetase during nodule development in lupin. Australian Journal of Plant Physiology 2:265–272
    [Google Scholar]
  25. Robertson J. G., Warburton M. P., Farnden K. J. F. 1975b; Induction of glutamate synthase during nodule development in lupin. FEBS Letters 55:33–37
    [Google Scholar]
  26. Roon R. J., Even H. L., Dunlop P., Larimore F. L. 1975; Methylamine and ammonia transport in Saccharomyces cerevisiae. . Journal of Bacteriology 122:502–509
    [Google Scholar]
  27. Stevenson R., Silver S. 1977; Methylammonium uptake by Escherichia coli: evidence for a bacterial NH4+ transport system. Biochemical and Biophysical Research Communications 75:1133–1139
    [Google Scholar]
  28. Stewart W. D. P., Rodgers G. A. 1977; The cyanophyte-hepatic symbiosis. II. Nitrogen fixation and the interchange of nitrogen and carbon. New Phytologist 78:459–471
    [Google Scholar]
  29. Stewart W. D. P., Rowell P., Aple S. K. 1977; Cellular physiology and the ecology of N2-fixing blue-green algae. In Recent Developments in Nitrogen Fixation pp. 287–307 Edited by Newton W. E., Postgate J. R., Rodriguez-Barreuco C. London: Academic Press;
    [Google Scholar]
  30. Stock J. B., Rauch B., Roseman S. 1977; Periplasmic space in Salmonella typhimurium and Escherichia coli. . Journal of Biological Chemistry 252:7850–7861
    [Google Scholar]
  31. Tubb R. S. 1976; Regulation of nitrogen fixation in Rhizobium spp. Applied and Environmental Micro-biology 32:483–488
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-1-29
Loading
/content/journal/micro/10.1099/00221287-128-1-29
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error