The resistance of Pseudomonas aeruginosa wild-type, uvr, pol and rec strains to ultraviolet (u.v.) light, X-rays and freezing and thawing was determined. An R plasmid, pPL1, which increased resistance of the wild-type, uvr, and pol but not rec strains to u.v. light, increased the resistance of only rec and pol mutants to X-rays and freezing and thawing. These findings reinforce the idea of DNA as a target in the organism for freeze-thaw stress and suggest that freeze-thaw-induced DNA damage might be similar to that produced by X-rays but different from that produced by u.v. light.
BeuchatL. R.1978; Injury and repair of Gramnegative bacteria with special consideration of the involvement of the cytoplasmic membrane. Advances in Applied Microbiology 23:219–248
BridgesB. A.1976; Survival of bacteria following exposure to ultraviolet light and ionizing radiations. Symposia of the Society for General Microbiology 26:183–208
BridgesB. A.,
Ashwood-SmithM. J.,
MunsonR. J.1969; Correlation of bacterial sensitivities to ionizing radiation and mild heating. Journal of General Microbiology 58:115–124
CalcottP. H.,
SteenbergenS. M.,
PettyR. S.1979a; The effect of freezing and thawing on the uptake and retention of amino acids in Escherichia coli
. FEMS Microbiology Letters 6:267–273
CalcottP. H.,
ZaborowskiC.,
LevineW. L.,
TruongN. H.1979b; Drug resistance plasmid (pPLl) mediated changes in the susceptibility of Pseudomonas aeruginosa to stress. FEMS Microbiology Letters 6:75–80
CoxC. S.,
HecklyR. J.1973; Effect of oxygen upon freeze-dried and freeze-thawed bacteria: viability and free-radical production. Canadian Journal of Microbiology 19:189–194
LeeS. K.,
CalcottP. H.,
MacleodR. A.1977; Relationship of cytochrome content to the sensitivity of bacteria to NaCl on freezing and thawing. Canadian Journal of Microbiology 23:413–419
LehrbachP.,
KungA. H.,
LeeB. T. O.1977a; Loss of ultraviolet light protection and enhanced ultraviolet light-induced mutability in Pseudomonas aeruginosa carrying mutant R plasmids. Journal of General Microbiology 101:135–141
LehrbachP.,
KungA. H.,
LeeB. T. O.,
JacobyG. B.1977b; Plasmid modification of radiation and chemical-mutagen sensitivity in Pseudomonas aeruginosa
. Journal of General Microbiology 98:167–176
LehrbachP. R.,
KungA. H. C.,
LeeB. T. O.1978; R plasmids which alter ultraviolet light- sensitivity and enhance ultraviolet light-induced mutability in Pseudomonas aeruginosa
. Journal of General Microbiology 108:119–123
LehrbachP.,
LeeB. T. O.,
DirckzeC.1979; Effect of repair deficiency and R plasmids on spontaneous and radiation-induced mutability in Pseudomonas aeruginosa
. Journal of Bacteriology 136:953–960
SatoM.,
TakahashiH.1970; Cold shock of bacteria. IV. Involvement of DNA ligase reaction in recovery of Escherichia coli from cold shock. Journal of General and Applied Microbiology 16:279–290
SedgwickS. G.,
BridgesB. A.1972; Evidence for indirect production of DNA strand scissions during mild heating of Escherichia coli
. Journal of General Microbiology 71:191–193
SongJ. C.,
CalcottP. H.1980; DNA damage and its repair in frozen-thawed Escherichia coli
. Third Semiannual Ohio Valley Tissue Culture Symposium, Hueston Woods, Ohio
WilliamsD. L.,
CalcottP. H.1980; The sensitivity of DNA repair mutants of Pseudomonas aeruginosa to freezing and thawing and the role of a UV repair plasmid in conferring resistance to stress. Third Semiannual Ohio Valley Tissue Culture Symposium, Hueston Woods, Ohio.