@article{mbs:/content/journal/micro/10.1099/00221287-128-1-189, author = "Yamada, Haruki and Ohshima, Yukio and Miyazaki, Toshio", title = "Heterogeneity and Antigenic Properties of Mannoheteroglycan from Absidia cylindrospora", journal= "Microbiology", year = "1982", volume = "128", number = "1", pages = "189-197", doi = "https://doi.org/10.1099/00221287-128-1-189", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-128-1-189", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "An antigenic fraction extracted from mycelium of Absidia cylindrospora contained several mannose-containing glycoproteins which differed in molecular weight and in their affinities to concanavalin A (Con A) and DEAE-Sephadex A-50. The fraction of the extract that bound to concanavalin A was separated into high-mannoproteins (minor fraction) and fucomannopeptides (major fraction). The former showed higher antibody-precipitating activity than the latter. Subfractions of the fucomannopeptides obtained by DEAE-Sephadex A-50 chromatography showed increased serological reactivity and an increased mannose to fucose molar ratio with increasing acidity, but neutral and acidic fractions of the mannoproteins showed the same degree of serological reactivity. The results indicate that the mannosecontaining antigenic fractions are highly heterogeneous and can be classified into high-mannose type (mol. wt 15000–35000) and fucomannan type (mol. wt 15000–70000).", }