1887

Abstract

In medium saturated with oxygen, the cyclopropane synthetase (unsaturated-phospholipid methyltransferase; EC 2.1.1.16) of was generally synthesized after the mid-exponential phase of growth. The enzyme could also be induced by rapidly limiting the oxygen supply, or by initiating respiration on nitrate or thiosulphate following an initial period of growth in a highly aerobic environment. In each of these ‘step-down’ situations the specific activity of cyclopropane synthetase rose to a maximum prior to the stationary phase of growth and subsequently decreased. The cyclopropane fatty acids, methylene hexadecanoic acid and methylene octadecanoic acid accumulated throughout exponential growth following the induction of the enzyme. During a 12 h period in the stationary phase there was little synthesis of either of the fatty acids, despite detectable cyclopropane synthetase activity in the cells, indicating that essentially all the fatty acid synthesis was complete prior to entering the stationary phase. When nitrate was used as a respiratory electron acceptor, a twofold increase in octadecenoic acid was observed, giving rise to an increase in methylene octadecanoic acid. This increase in octadecenoic acid was not apparent in mutants unable to respire on nitrate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-1-177
1982-01-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/1/mic-128-1-177.html?itemId=/content/journal/micro/10.1099/00221287-128-1-177&mimeType=html&fmt=ahah

References

  1. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Cronan J. E. Jr 1968; Phospholipid alterations during growth of Escherichia coli. Journal of Bacteriology 95:2054–2061
    [Google Scholar]
  3. Cronan J. E. Jr Nunn W. D., Bachelor J. G. 1974; Studies on the biosynthesis of cyclopropane fatty acids in Escherichia coli. . Biochimica et biophysica acta 348:63–75
    [Google Scholar]
  4. Finnerty W. R., Makula R. A. 1975; Microbial lipid metabolism. CRC Critical Reviews in Microbiology 4:1–40
    [Google Scholar]
  5. Halper L. A., Norton S. J. 1975; Regulation of cyclopropane fatty acid biosynthesis by variations in enzyme activities. Biochemical and Biophysical Research Communications 62:683–688
    [Google Scholar]
  6. Hancock I. C., Meadow P. M. 1969; The extractable lipids of Pseudomonas aeruginosa. . Biochimica et biophysica acta 187:366–379
    [Google Scholar]
  7. Herbert D. 1965; Microbial respiration and oxygen tension. Journal of General Microbiology 41:viii–ix
    [Google Scholar]
  8. Hunt A. L. 1959; Purification of the nicotinic acid hydroxylase system of Pseudomonas fluorescens KB1. Biochemical Journal 72:1–7
    [Google Scholar]
  9. Jacques N. A., Hunt A. L. 1980; Studies on cyclopropane fatty acid synthesis. Effect of carbon source and oxygen tension on cyclopropane fatty acid synthetase activity in Pseudomonas denitri- ficans. . Biochimica et biophysica acta 619:453–470
    [Google Scholar]
  10. Klopfenstein W. E. 1971; On methylation of unsaturated acids using boron trihalide-methanol reagents. Journal of Lipid Research 12:773–776
    [Google Scholar]
  11. Nason A., Evans H. J. 1963; Nitrate reductase from Neurospora. . Methods in Enzymology 2:411–412
    [Google Scholar]
  12. Pléchaud M., Plug J., Plchinoty F., Azoulay E., Le Minor L. 1967; Mutations affectant la nitrate-réductase A et d’autres enzymes bactériennes d’oxydoréduction. Annales de l’Institut Pasteur 112:24–37
    [Google Scholar]
  13. Taylor F., Cronan J. E. Jr 1976; Selection and properties of Escherichia coli mutants defective in the synthesis of cyclopropane fatty acids. Journal of Bacteriology 125:518–523
    [Google Scholar]
  14. Vulliet P., Markey S. P., Tornabene T. G. 1974; Identification of methoxyester artifacts produced by methanoiic-HC1 solvolysis of the cyclopropane fatty acids of the genus Yersinia. . Biochimica et biophysica acta 348:299–301
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-1-177
Loading
/content/journal/micro/10.1099/00221287-128-1-177
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error