1887

Abstract

N-fixing possesses an H-uptake hydrogenase activity capable of supporting H-dependent acetylene reduction by whole cells starved of carbon metabolites. H did not support acetylene reduction in carbon-sufficient bacteria. although carbon substrates did not inhibit H-dependent respiration. H-dependent respiration was extremely O sensitive and could not usually protect nitrogenase against inhibition by O. H partly inhibited nitrogenase activity at sub-optimal O concentrations: this may be because H-dependent respiration has a greater affinity for O but is less efficiently coupled to ATP production than is carbon-dependent respiration.

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1982-01-01
2024-04-19
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References

  1. Berlier Y. M., Lespinat P. A. 1980; Mass-spectroscopic kinetic studies of the nitrogenase and hydrogenase activities in in vivo cultures of Azospirillum brasilense, Sp 7. Archives of Microbiology 125:67–72
    [Google Scholar]
  2. Bothe H., Tennigkeit J., Eisbrenner G. 1977; The utilization of molecular hydrogen by the blue-green alga Anabaena cylindrica. Archives of Microbiology 114:43–49
    [Google Scholar]
  3. Burgess B. K., Wherland S., Stiefel E. I., Newton W. E. 1980; HD formation by nitrogenase: a probe for N2 reduction intermediates. In Molybdenum Chemistry of Biological Significance pp. 73–84 Edited by Newton W. E., Otsuka S. New York: Plenum Press;
    [Google Scholar]
  4. Chan Y. K., Nelson L. M., Knowles R. 1980; Hydrogen metabolism of Azospirillum brasilense in nitrogen-free medium. Canadian Journal of Microbiology 26:1126–1131
    [Google Scholar]
  5. Dixon R. O. D. 1972; Hydrogenase in legume root nodule bacteroids: occurrence and properties. Archiv für Mikrobiologie 85:193–201
    [Google Scholar]
  6. Emerich D. W., Ruiz-Argûeso T., Russell S. A., Evans H. J. 1979; Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids. Journal of Bacteriology 137:153–160
    [Google Scholar]
  7. Laane C., Haaker H., Veeger C. 1979; On the efficiency of oxidative phosphorylation in membrane vesicles of Azotobacter vinelandii and of Rhizobium leguminosarum bacteroids. European Journal of Biochemistry 97:369–377
    [Google Scholar]
  8. Okon Y., Albrecht S. L., Burris R. W. 1976; Some factors affecting the growth and nitrogen fixation of Spirillum lipoferum. Journal of Bacteriology 127:1248–1254
    [Google Scholar]
  9. Stephan M., Pedrosa F. O., Dobereiner J. 1981; Physiological studies with Azospirillum Sp 7. In Associative N2 fixation (in the Press) Edited by Vose P. B., Ruschel A. P. Florida: CRC Press;
    [Google Scholar]
  10. Tarrand J. J., Krieg N. R., Dobereiner J. 1978; A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov.and two species Azospirillum lipoferum (Beijerinck) comb. nov.Azospirillum brasilense sp. nov. Canadian Journal of Microbiology 24:967–980
    [Google Scholar]
  11. Walker C. C., Yates M. G. 1978; The hydrogen cycle in nitrogen-fixing Azotobacter chroococcum. Biochimie 60:225–232
    [Google Scholar]
  12. Walker C. C., Partridge C. D. P., Yates M. G. 1981; The effect of nutrient limitation on hydrogen production by nitrogenase in continuous cultures of Azotobacter chroococcum. Journal of General Microbiology 124:317–327
    [Google Scholar]
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