SUMMARY: N-fixing possesses an H-uptake hydrogenase activity capable of supporting H-dependent acetylene reduction by whole cells starved of carbon metabolites. H did not support acetylene reduction in carbon-sufficient bacteria. although carbon substrates did not inhibit H-dependent respiration. H-dependent respiration was extremely O sensitive and could not usually protect nitrogenase against inhibition by O. H partly inhibited nitrogenase activity at sub-optimal O concentrations: this may be because H-dependent respiration has a greater affinity for O but is less efficiently coupled to ATP production than is carbon-dependent respiration.


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