1887

Abstract

The immunological basis for resistance of certain strains of to the bactericidal action of normal human serum was studied by investigating the potential role of factors which are known to interfere with each of the sequential steps that result in immune lysis of Gram-negative bacteria. Strains of were characterized as serum-sensitive (ser) or serum-resistant (ser) on the basis of their sensitivity to lysis by the sera of six normal individuals. Neither intrinsic resistance to the lytic action of activated human complement nor inaccessibility of the cell membrane to C5b accounted for ser. Outer membrane lipopolysaccharide (LPS) was the target antigen for lytic antibody in normal human sera. The gross chemical composition and molecular size of the LPS of the strains were heterogeneous and no consistent patterns of differences between those extracted from ser and from ser strains were found. Neither IgA nor IgG ‘blocking’ antibody in normal human serum was responsible for ser. We conclude that ser results from the absence from the LPS of the strains of antigenic loci for the lytic antibody in most normal human sera, or, expressed as a function of the host, the absence from the sera of most normal humans of lytic antibody directed against LPS antigenic loci for immune lysis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-1-13
1982-01-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/1/mic-128-1-13.html?itemId=/content/journal/micro/10.1099/00221287-128-1-13&mimeType=html&fmt=ahah

References

  1. Bertram M. A., Griffiss J. MCL., Broud D. D. 1976; Response to antigenic determinants of Neisseria meningitidis lipopolysaccharide investigated with a new radioactive antigen-binding assay. Journal of Immunology 116:842–846
    [Google Scholar]
  2. Bhatti T., Chambers R. E., Clamp J. R. 1970; The gas chromatographic properties of biologically important N-acetylglucosamine derivatives, monosaccharides, disaccharides, trisaccharides, tetrasaccharides and pentasaccharides. Biochimica et biophysica acta 222:339–347
    [Google Scholar]
  3. Brooks G. F., Israel K. S., Peterson B. H. 1976; Bactericidal and opsonic activity against Neisseria gonorrhoeae in sera from patients with disseminated gonococcal infection. Journal of Infectious Diseases 134:450–462
    [Google Scholar]
  4. Frank M. M. 1979; The complement system in host defense and inflammation. Reviews of Infectious Diseases 1:483–501
    [Google Scholar]
  5. Galanos C. 1975; Physical state and biological activity of lipopoly saccharides.Toxicity and immunogenicity of the Lipid A component. Zeitschrift für Immunitätsforschung und experimentelle Therapie 149:214–229
    [Google Scholar]
  6. Galanos C., Luderitz O., Westphal O. 1969; A new method for the extraction of R lipopolysaccharides. European Journal of Biochemistry 9:245–249
    [Google Scholar]
  7. Glynn A. A., Ward M. E. 1970; Nature and heterogeneity of the antigens of Neisseria gonorrhoeae involved in the serum bactericidal reaction. Infection and Immunity 2:162–168
    [Google Scholar]
  8. Goldschneider I., Gotschlich E. C., Artenstein M. S. 1969; Human immunity to the meningococcus. I. The role of humoral antibodies. Journal of Experimental Medicine 129:1307–1326
    [Google Scholar]
  9. Griffiss J. McL., Bertram M. A. 1977; Immunoepidemiology of meningococcal disease in military recruits. II. Blocking of serum bactericidal activity by circulating IgA early in the course of invasive disease. Journal of Infectious Diseases 136:733–739
    [Google Scholar]
  10. Griffiss J. McL., Goroff D. K. 1981; Immunological cross-reaction between a naturally occurring galactan, agarose, and an LPS locus for immune lysis of Neisseria meningitidis by human sera. Clinical and Experimental Immunology 43:20–27
    [Google Scholar]
  11. Griffiss J. McL., Bertram M. A., Broud D. D. 1978; Separation and purification of immunoglobulins M, A and G from small volumes of human sera by a continuous, in-line chromatographic process. Journal of Chromatography 156:121–130
    [Google Scholar]
  12. Hook W. A., Muschel L. 1964; Anticomplementary effects and complement activity of human sera. Proceedings of the Society for Experimental Biology and Medicine 117:292–297
    [Google Scholar]
  13. Kellogg D. S., Peacock W. L., Deacon W. E., Brown L., Pirkle C. I. 1963; Neisseria gonorrhoeae. I. Virulence genetically linked to clonal variation. Journal of Bacteriology 85:1274–1279
    [Google Scholar]
  14. Luderitz O., Westphal O. 1966; The significance of enterobacterial mutants for the chemical investigation of their cell-wall polysaccharides. Angewandte Chemie (International Edition) 5:198–210
    [Google Scholar]
  15. Mccutchan J. A., Katzenstein D., Norquist D., Chikami G., Wunderlich A., Braude A. I. 1978; Role of blocking antibody in disseminating gonococcal infection. Journal of Immunology 121:1884–1888
    [Google Scholar]
  16. Nicholson A., Lepow I. H. 1979; Host defense against Neisseria meningitidis requires a complement-dependent bactericidal activity. Science 205:298–299
    [Google Scholar]
  17. Osborn M. J. 1963; Studies on the Gram-negative cell wall. I. Evidence for the role of 2-keto-3- deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium. Proceedings of the National Academy of Sciences of the United States of America 50:499–506
    [Google Scholar]
  18. Petersen B. H., Graham J. A., Brooks G. F. 1976; Human deficiency of the eighth component of complement: the requirement of C8 for serum Neisseria gonorrhoeae bactericidal activity. Journal of Clinical Investigation 57:283–290
    [Google Scholar]
  19. Pier G. B., Sidberry H. F., Zolyomi S., Sadoff J. C. 1978; Isolation and characterization of a high-molecular-weight polysaccharide from the slime of Pseudomonas aeruginosa. Infection and Immunity 22:908–918
    [Google Scholar]
  20. Reynolds B. L., Pruul H. 1971; Protective role of smooth lipopolysaccharide in the serum bactericidal reaction. Infection and Immunity 4:764–771
    [Google Scholar]
  21. Reynolds B. L., Rowley D. 1969; Sensitization of complement-resistant bacterial strains. Nature; London: 2211259–1261
    [Google Scholar]
  22. Reynolds B. L., Rother U. A., Rother K. O. 1975; Interaction of complement components with a serum-resistant strain of Salmonella typhimurium. Infection and Immunity 11:944–948
    [Google Scholar]
  23. Rice P. A., Mccormack W. M., Kasper D. L. 1980; Natural serum bactericidal activity against Neisseria gonorrhoeae isolates from disseminated, locally invasive, and uncomplicated disease. Journal of Immunology 124:2105–2109
    [Google Scholar]
  24. Roe J. H. 1955; The determination of sugar in blood and spinal fluid with the anthrone reagent. Journal of Biological Chemistry 212:335–343
    [Google Scholar]
  25. Schneider H., Griffiss J. McL., Williams G. D., Pier G. B. 1978; Noncorrelation of Neisseria gonorrhoeae serum sensitivity and serum resistance with molecular weight of gonococcallipopoly-saccharides. In Immunobiology of Neisseria gonorrhoeae pp. 196–198 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Edited by Washington:: American Society for Microbiology.;
    [Google Scholar]
  26. Schoolnik G. K., Buchanan T. M., Holmes K. K. 1976; Gonococci causing disseminated gonococcal infections are resistant to the bactericidal action of normal human sera. Journal of Clinical Investigation 58:1163–1173
    [Google Scholar]
  27. Tramont E. C., Sadoff J. C., Wilson C. 1977; Variability of the lytic susceptibility of Neisseria gonorrhoeae to human sera. Journal of Immunology 118:1843–1851
    [Google Scholar]
  28. Ward M. E., Lambden P. R., Heckels J. E., Watt P. J. 1978; The surface properties of Neisseria gonorrhoeae: determinants of susceptibility to antibody complement killing. Journal of General Microbiology 108:205–212
    [Google Scholar]
  29. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides. Methods in Carbohydrate Chemistry 5:83–91
    [Google Scholar]
  30. White L., Kellogg D. S. 1965; Neisseria gonorrhoeae identification in direct smears by a fluorescent antibody-counterstain method. Applied Microbiology 13:171–174
    [Google Scholar]
  31. Zollinger W. D., Mandrell R. E. 1977; Outer membrane protein and lipopolysaccharide serotyping of Neisseria meningitidis by inhibition of a solid phase radioimmunoassay. Infection and Immunity 18:424–433
    [Google Scholar]
  32. Zollinger W. D., Mandrell R. E., Griffiss J. McL., Altieri P. L., Bertram S. 1979; Complex of meningococcal group B polysaccharide and type 2 outer membrane protein immunogenic in man. Journal of Clinical Investigation 63:836–848
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-1-13
Loading
/content/journal/micro/10.1099/00221287-128-1-13
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error