1887

Abstract

Acid methanolysates of 104 strains of coryneform and related bacteria were examined for mycolic acids by thin-layer chromatography and mass spectrometry. The mycolic acids of strains were not uniform in size and structure, and considerable variation existed within the approximate limiting range of C to C. Two species, and were particularly distinctive; the former had exceptionally low molecular weight mycolic acids (C to C), whereas the latter contained major amounts of mycolates with a side-chain possessing an odd number of carbon atoms. contained mycolic acids of a similar size (C to C) to those of true corynebacteria. The mycolates of organisms assigned to the genus were generally larger (C to C), although a clear distinction between true corynebacteria and rhodococci cannot presently be made by analysis of mycolic acids alone. Strains labelled and contained mycolic esters with molecular weights ‘intermediate’ in size between those of true corynebacteria and rhodococci. The mycolic acid data correlate well with other major trends in coryneform taxonomy and support earlier suggestions that they are of value in the classification of the group.

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1982-01-01
2021-05-12
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References

  1. Abe S., Takayama K., Kinoshita S. 1967; Taxonomical studies on glutamic acid-producing bacteria. Journal of General and Applied Microbiology 13:279–301
    [Google Scholar]
  2. Alshamaony L., Goodfellow M., Minnikin D. E. 1976a; Free mycolic acids as criteria in the classification of Nocardia and the ‘rhodochrous’ complex. Journal of General Microbiology 92:188–199
    [Google Scholar]
  3. Alshamaony L., Goodfellow M., Minnikin D. E., Mordarska H. 1976b; Free mycolic acids as criteria in the classification of Gordona and the ‘rhodochrous’ complex . Journal of General Micro biology 92:183–187
    [Google Scholar]
  4. Alshamaony L., Goodfellow M., Minnikin D. E., Bowden G. H., Hardie J. M. 1977; Fatty and mycolic acid composition of Bacterionema matruchotii and related organisms. Journal of General Microbiology 98:205–213
    [Google Scholar]
  5. Barksdale L. 1970; Corynebacterium diphtheriae and its relatives. Bacteriological Reviews 34:378–422
    [Google Scholar]
  6. Barksdale L., Lanéelle M. A., Pollice M. C., Asselineau J., Welby M., Norgard M. 1979; Biological and chemical basis for the reclassification of Microbacterium flavum Orla-Jensen as Corynebacterium flavescens nom. rev. International Journal of Systematic Bacteriology 29:222–233
    [Google Scholar]
  7. Beaman B. L., Kris K. S., Lanéelle M.-A., Barksdale L. 1974; Chemical characterization of organisms isolated from leprosy patients. Journal of Bacteriology 117:1320–1324
    [Google Scholar]
  8. Bousfield I. J., Goodfellow M. 1976; The ‘rhodochrous’ complex and its relationship with allied taxa. In The Biology of the Nocardiae pp. 39–85 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  9. Campbell I. M., Naworal J. 1969; Mass spectral discrimination between monoenoic and cyclopropanoid, and between normal, iso and anteiso fatty acid methyl esters. Journal of Lipid Research 10:589–592
    [Google Scholar]
  10. Collins M. D., Jones D. 1980; Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. Journal of Applied Bacteriology 48:459–470
    [Google Scholar]
  11. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of mena- quinones in actinomycetes and corynebacteria. Journal of General Microbiology 100:221–230
    [Google Scholar]
  12. Collins M. D., Goodfellow M., Minnikin D. E. 1979a; Isoprenoid quinones in the classification of coryneform and related bacteria. Journal of General Microbiology 110:127–136
    [Google Scholar]
  13. Collins M. D., Jones D., Goodfellow M., Minnikin D. E. 1979b; Isoprenoid quinone composition as a guide to the classification of Listeria, Brochothrix, Erysipelothrix and Caryophanon . Journal of General Microbiology 111:453–457
    [Google Scholar]
  14. Collins M. D., Goodfellow M., Minnikin D. E. 1980; Fatty acid, isoprenoid quinone and polar lipid composition in the classification of Curto- bacterium and related taxa. Journal of General Microbiology 118:29–37
    [Google Scholar]
  15. Corina D. L., Sesardic D. 1980; Profile analysis of total mycolic acids from skin corynebacteria and from named Corynebacterium strains by gas-liquid chromatography and gas-liquid chromatography/ mass spectrometry. Journal of General Microbiology 116:61–68
    [Google Scholar]
  16. Crombach W. H. J. 1972; DNA base composition of soil arthrobacters and other coryneforms from cheese and sea fish. Antonie van Leeuwenhoek 29:344–358
    [Google Scholar]
  17. Crombach W. H. J. 1978; Caseobacter poly-morphus gen. nov., sp. nov., a coryneform bacterium from cheese. International Journal of Systematic Bacteriology 28:354–366
    [Google Scholar]
  18. Diara A., Pudles J. 1959; Sur les lipides de Corynebacterium ovis . Bulletin de la Société de chimie biologique 41:481–486
    [Google Scholar]
  19. Etémadi A. H. 1967; The use of pyrolysis gas chromatography and mass spectroscopy in the study of the structure of mycolic acids. Journal of Gas Chromatography 5:447–456
    [Google Scholar]
  20. Goodfellow M., Alderson G. 1977; The actinomycete-genus Rhodococcus: a home for the ‘rhodochrous’ complex . Journal of General Microbiology 100:99–122
    [Google Scholar]
  21. Goodfellow M., Collins M. D., Minnikin D. E. 1976; Thin-layer chromatographic analysis of mycolic acid and other long-chain components in whole-organism methanolysates of coryneform and related taxa. Journal of General Microbiology 96:351–358
    [Google Scholar]
  22. Goodfellow M., Orlean P. A. B., Collins M. D., Alshamaony L., Minnikin D. E. 1978; Chemical and numerical taxonomy of strains received as Gordona aurantiaca . Journal of General Microbiology 109:57–68
    [Google Scholar]
  23. Jones D. 1975; A numerical taxonomic study of coryneform and related bacteria. Journal of General Microbiology 87:52–96
    [Google Scholar]
  24. Jones D. 1978; An evaluation of the contributions of numerical taxonomic studies to the classification of coryneform bacteria. In Coryneform Bacteria pp. 13–46 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  25. Keddie R. M., Bousfield I. J. 1980; Cell wall composition in the classification and identification of coryneform bacteria. In Microbiological Classification and Identification pp. 167–188 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  26. Keddie R. M., Cure G. L. 1977; The cell wall composition and distribution of free mycolic acids in named strains of coryneform bacteria and in isolates from various natural sources. Journal of Applied Bacteriology 42:229–252
    [Google Scholar]
  27. Keddie R. M., Cure G. L. 1978; Cell wall composition of coryneform bacteria. In Coryneform Bacteria pp. 47–83 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  28. Krasilnikov N. A., Koronelli T. V., Rozynov B. A. 1972; Aliphatic and mycolic acids of Mycobacterium paraffinicum . Mikrobiologiya 41:808–812
    [Google Scholar]
  29. Krasilnikov N. A., Koronelli T. V., Rozynov B. A., Kalyuzhnaya T. V. 1973; Mycolic acids of pigmented paraffin-oxidizing mycobacteria. Mikrobiologiya 42:240–243
    [Google Scholar]
  30. Lechevalier M. P., Lechevalier H. A., Horan A. C. 1973; Chemical characteristics and the classification of nocardiae. Canadian Journal of Microbiology 19:965–972
    [Google Scholar]
  31. Maurice M. T., Vacheron M. J., Michel G. 1971; Isolement d’acides nocardiques de plusiers especes de Nocardia . Chemistry and Physics of Lipids 7:9–18
    [Google Scholar]
  32. Minnikin D. E., Goodfellow M. 1976; Lipid composition in the classification and identification of nocardiae and related taxa. In The Biology of the Nocardiae pp. 160–219 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  33. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid-fast bacteria. In Microbiological Classification and Identification pp. 189–256 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  34. Minnikin D. E., Alshamaony L., Goodfellow M. 1975; Differentiation of Mycobacterium, Nocardia and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. Journal of General Microbiology 88:200–204
    [Google Scholar]
  35. Minnikin D. E., Goodfellow M., Collins M. D. 1978; Lipid composition in the classification and identification of coryneform and related taxa. In Coryneform Bacteria pp. 85–160 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  36. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. Journal of Applied Bacteriology 47:87–95
    [Google Scholar]
  37. Okazaki H., Sugino H., Kanzaki T., Fukuda H. 1969; l-Glutamic acid fermentation. VI. Structure of a sugar lipid produced by Brevibacterium thiogenitalis . Agricultural and Biological Chemistry 33:764–770
    [Google Scholar]
  38. Schleifer K. H., Kandler O. 1972; Peptidogly-can types of bacterial cell walls and their taxonomic implications. Bacteriologial Reviews 36:407–477
    [Google Scholar]
  39. Senn M., Ioneda T., Pudles J., Lederer E. 1967; Spectrometrie de masse de glycolipids I. Structure du ‘cord factor’ de Corynebacterium diphtheriae . European Journal of Biochemistry 1:353–356
    [Google Scholar]
  40. Skerman V. B. D., Mcgowan V., Sneath P. H. A. editors 1980; Approved Lists of Bacterial Names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  41. Starr M. P., Mandel M., Murata M. 1975; The phytopathogenic coryneform bacteria in the light of DNA base composition and DNA-DNA segmental homology. Journal of General and Applied Microbiology 21:13–26
    [Google Scholar]
  42. Suzuki T., Tanaka K., Matsubara I., Kinoshita S. 1969; Trehalose lipid and ʱ-branched-β-hydroxy fatty acid formed by bacteria grown on n-alkanes. Agricultural and Biological Chemistry 33:1619–1627
    [Google Scholar]
  43. Welby-Gieusse M., Lanéelle M. A., Asselineau J. 1970; Structure des acides corynomycoliques de Corynebacterium hofmanii et leur implication biogénétique. European Journal of Biochemistry 13:164–167
    [Google Scholar]
  44. Yamada Y., Inouye G., Tahara Y., Kondo K. 1976; The menaquinone system in the classification of coryneform and nocardioform bacteria and related organisms. Journal of General and Applied Microbiology 22:203–214
    [Google Scholar]
  45. Yano I., Saito K. 1972; Gas chromatographic and mass spectrometrie analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans . FEBS Letters 23:352–356
    [Google Scholar]
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