1887

Abstract

sp. strain E4 was grown in continuous-flow culture with either monochloroacetate (MCA) or 2-monochloropropionate (2MCPA) as the growth-limiting substrate. In contrast to previous observations made for this organism grown in closed culture on 2MCPA, a third dehalogenase was detected under certain growth conditions, as well as dehalogenases I and II. The response of sp. strain E4, in terms of its enzyme complement, varied depending on the organism’s growth rate and the nature of the growth-limiting substrate. With MCA as the growth-limiting substrate, dehalogenases II and III were only detected in slowly growing organisms. With 2MCPA as growth-limiting substrate, the responses were more variable and complex. The overall dehalogenase activities also depended on the growth rate and substrate limitation, with higher dehalogenation rates found in organisms growing slowly with MCA limitation compared with 2MCPA limitation. In fast growing organisms the relative rates of dehalogenation were reversed for the two limitations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-2-399
1981-12-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/2/mic-127-2-399.html?itemId=/content/journal/micro/10.1099/00221287-127-2-399&mimeType=html&fmt=ahah

References

  1. Almengor-Hecht M.L., Bull A.T. 1978; Continuous-flow enrichment of a strain of Erwinia carotovora having specificity for highly methylated pectin>. Archives of Microbiology 119:163–166
    [Google Scholar]
  2. Bolton P.G., Dean A.C.R. 1972; Phosphatase synthesis in Klebsiella (Aerobacter) aerogenes growing in continuous culture. Biochemical Journal 127:87–96
    [Google Scholar]
  3. Bull A.T., Brown C.M. 1979; Continuous culture applications to microbial biochemistry. In International Review of Biochemistry 21 Microbial Biochemistry pp. 177–226 Quayle J.R. Edited by Baltimore: University Park Press;
    [Google Scholar]
  4. Bull A.T., Senior E., Slater J.H. 1976; Concerning the evolution of metabolic capabilities in bacteria: Dalapon (2,2-dichloropropionic acid) metabolism by Pseudomonas putida>. Proceedings of the Society for General Microbiology 4:39
    [Google Scholar]
  5. Bushell M.E., Bull A.T. 1981; Anaplerotic metabolism of Aspergillus nidulans and its effect on biomass synthesis in carbon limited chemostats. Archives of Microbiology 128:282–287
    [Google Scholar]
  6. Clarke P.H., Lilly M.D. 1969; The regulation of enzyme synthesis during growth. Symposia of the Society for General Microbiology 19:113–159
    [Google Scholar]
  7. Dean A.C.R. 1972; Influence of environment on the control of enzyme synthesis. Journal of Applied Chemistry and Biotechnology 22:245–259
    [Google Scholar]
  8. Goldman P., Milne G.W.A., Keister D.B. 1968; Carbon-halogen bond cleavage. III. Studies on bacterial halidohydrolases. Journal of Biological Chemistry 243:428–434
    [Google Scholar]
  9. Gornall A.G., Bardawill C.J., David M.M. 1949; Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  10. Hardman D.J., Slater J.H. 1981; Dehalogenases in soil bacteria. Journal of General Microbiology 123:117–128
    [Google Scholar]
  11. Jensen H.L. 1960; Decomposition of chloroacetates and chloropropionates by bacteria. Acta agriculturae scandinavica 10:83–103
    [Google Scholar]
  12. Little M., Williams P.A. 1971; A bacterial halidohydrolase: its purification by specific amino acid reagents. European Journal of Biochemistry 21:99–109
    [Google Scholar]
  13. Slater J.H., Lovatt D., Weightman A.J., Senior E., Bull A.T. 1979; The growth of Pseudomonas putida on chlorinated aliphatic acids and its dehalogenase activity. Journal of General Microbiology 114:125–136
    [Google Scholar]
  14. Weightman A.J., Slater J.H. 1980; Selection of Pseudomonas putida strains with elevated dehalogenase activities by continuous culture growth on chlorinated alkanoic acids. Journal of General Microbiology 121:187–193
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-2-399
Loading
/content/journal/micro/10.1099/00221287-127-2-399
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error