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Three cutaneous propionibacteria - Propionibacterium acnes, P. avidum and P. granulosum - were grown in chemostats using semi-synthetic medium with various concentrations of glucose. Biomass rose with increasing glucose concentration up to 0·3–0·4% (w/v) and then remained constant. Propionibacterium granulosum had both a low yield and μ max when grown in the absence of glucose suggesting that this organism is essentially ‘saccharolyti’ in its nutrition. In contrast, P. acnes and P. avidum had higher growth yields than P. granulosum and showed their highest μ max values in the absence of glucose. Extracellular lipase, hyaluronidase (hyaluronate lyase) and acid phosphatase activities varied with different glucose concentrations, but in all cases were lowest at the highest glucose concentration tested (0·5%, w/v).
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