1887

Abstract

Three cutaneous propionibacteria - and - were grown in chemostats using semi-synthetic medium with various concentrations of glucose. Biomass rose with increasing glucose concentration up to 0·3–0·4% (w/v) and then remained constant. had both a low yield and when grown in the absence of glucose suggesting that this organism is essentially ‘saccharolyti’ in its nutrition. In contrast, and had higher growth yields than and showed their highest values in the absence of glucose. Extracellular lipase, hyaluronidase (hyaluronate lyase) and acid phosphatase activities varied with different glucose concentrations, but in all cases were lowest at the highest glucose concentration tested (0·5%, w/v).

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1981-12-01
2021-05-12
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References

  1. Chen P.S., Toribara T.Y., Warner H. 1956; Microdetermination of phosphorus.. Analytical Chem istry 28:1756–1758
    [Google Scholar]
  2. Eaves G., Greenman J., Holland K.T. 1979; Decay rates of exocellular enzymes produced by bacteria - correction of production rates in chemo-stat cultures.. FEMS Microbiology Letters 6:333–336
    [Google Scholar]
  3. Ferguson D.A., Cummins C.S. 1978; Nutritional requirements of anaerobic coryneforms.. Journal of Bacteriology 135:858–867
    [Google Scholar]
  4. Greenman J., Cunliffe W.J., Holland K.T. 1979; The effect of nutritional changes on production of exocellular, biologically active substances by Propionibacterium acnes grown on a synthetic medium.. Journal of Investigative Dermatology 70:228 (abstract).
    [Google Scholar]
  5. Hassing G.S. 1971; Partial purification and some properties of lipase from Corvnebacterium acnes.. Biochimica et biophysica acta 242:381–394
    [Google Scholar]
  6. Holland K.T., Cunliffe W.J., Roberts C.D. 1978; The role of bacteria in acne - a newapproach.. Clinical and Experimental Dermatology 3:253–257
    [Google Scholar]
  7. Holland K.T., Cunliffe W.J., Roberts C.D. 1979; Growth of Propionibacterium acnes on synthetic medium: growth yields and exoenzyme production.. Journal of Applied Bacteriology 47:383–394
    [Google Scholar]
  8. Huggett A.St.G., Nixon D.A. 1957; Enzymic determination of blood glucose.. Biochemical Journal 66:P12
    [Google Scholar]
  9. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1979; Purification and partial characterization of hyaluronate lyase (EC 4.2.99.1) from Propionibacterium acnes.. Journal of General Microbiology 115:411–418
    [Google Scholar]
  10. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1980; Purification and partial characterization of an acid phosphatase (EC 3.1.3.2) produced by Propionibacterium acnes.. Journal of General Microbiology 118:59–65
    [Google Scholar]
  11. Ingham E., Holland K.T., Gowland G., Cunliffe W.J. 1981; Partial purification and characterization of lipase (EC 3.1.1.3) from Propionibacterium acnes.. Journal of General Microbiology 124:393–401
    [Google Scholar]
  12. Johnson J.L., Cummins C.S. 1972; Cell wall composition and deoxyribonucleic acid similarities among the anaerobic coryneforms, classical pro-pionibacteria, and strains of Arachnia propionica.. Journal of Bacteriology 109:1047–1066
    [Google Scholar]
  13. Kellum R.E., Strangfeld K., Ray L.F. 1970; Acne vulgaris. Studies in pathogenesis: triglyceride hydrolysis by Corynebacterium acnes in vitro.. Archives of Dermatology 101:41–47
    [Google Scholar]
  14. Magasanik B. 1961; Cellular regularity mechanisms.. Cold Spring Harbor Symposia on Quantitative Biology 26:249–256
    [Google Scholar]
  15. Marples R.R., McGinley K.J. 1974; Corynebacterium acnes and other anaerobic diphtheroids from human skin.. Journal of Medical Microbiology 7:349–357
    [Google Scholar]
  16. McGinley K.J., Webster G.F., Leyden J.J. 1978; Regional variations of cutaneous propioni-bacteria.. Applied and Environmental Microbiology 35:62–66
    [Google Scholar]
  17. Ministry of Agriculture Fisheries and Food 1973 The Analysis of Agricultural Material. Technical Bulletin 27: London:: HMSO.;
    [Google Scholar]
  18. Puhvel S.M., Reisner R.M. 1972; The production of hyaluronidase (hyaluronate lyase) by Corynebacterium acnes.. Journal of Investigative Dermatology 58:66–70
    [Google Scholar]
  19. Rebello T., Hawk J.L.M. 1978; Skin surface glycerol levels in acne vulgaris.. Journal of Investigative Dermatology 70:352–354
    [Google Scholar]
  20. Tempest D.W. 1970; The continuous cultivation of micro-organisms. 1. Theory of the chemostat.. Methods in Microbiology 2:259–276
    [Google Scholar]
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