1887

Abstract

Of seven strains of examined, four contained covalently closed circular DNA molecules. The bacteriocinogenic strain NCIB 7423 carried two plasmids: pCB101 of molecular mass 3·9 megadaltons (5·6 × 10 bases) and pCB102 of molecular mass 5·2 megadaltons (7·4 × 10 bases). Plasmid pCB101 was not restricted by RI which cleaved pCB102 at two sites; both plasmids were restricted by dIII. The three other plasmid-bearing strains of (SA1, SA11 and NCTC 6084) each carried a single plasmid of molecular mass 4·3 megadaltons (6·2 × 10 bases) with a single cleavage site for RI and four sites for dIII, the fragments so produced from the three plasmids being indistinguishable on agarose gel electrophoretograms. It was concluded that the three strains harboured a common plasmid - pCB103.

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1981-12-01
2021-05-15
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References

  1. Blaschek H.P., Solberg M. 1980; Plasmid mediated caseinase activity in Clostridium perfringens ATCC 3626 TYPE B. Abstracts of the Annual Meeting of the American Society for Microbiology Abstract 1268
    [Google Scholar]
  2. Brefort G., Magot M., Ionesco H., Sebald M. 1977; . Characterisation and transferability of Clostridium perfringens plasmids. Plasmid 1:52–66
    [Google Scholar]
  3. Brefort G., Magot M., Ionesco H., Sebald M. 1978; . Characterisation and transferability of Clostridium perfringens plasmids. In Microbiology-1978 pp. 242–245 Schlessinger D. Edited by Washington: American Society for Microbiology;
    [Google Scholar]
  4. Clarke D.J., Robson R.M., Morris J.G. 1975; Purification of two Clostridium bacteriocins by procedures appropriate to hydrophobic proteins. Antimicrobial Agents and Chemotherapy 7:256–264
    [Google Scholar]
  5. Colman A., Byers M.J., Primrose S.B., Lyons A. 1978; Rapid purification of plasmid DNAs by hydroxyapatite chromatography. European Journal of Biochemistry 91:303–310
    [Google Scholar]
  6. Currier T.C., Nester E.W. 1976; Isolation of covalently closed circular DNA of high molecular weight from bacteria. Analytical Biochemistry 76:431–441
    [Google Scholar]
  7. Davis R.W., Simon M., Davidson N. 1971; Electron microscopic heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  8. Dobrista A.P., Dobrista S.V., Tanyashin V.I. 1978; Isolation and characterisation of plasmid from Bacillus brevis var. GB cells. Molecular and General Genetics 164:195–204
    [Google Scholar]
  9. Duncan C.L., Rokos E.A., Christenson C.M. 1978; Multiple plasmids in different toxigenic types of Clostridium perfringens: possible control of beta toxin production. In Microbiology-1978 pp. 246–248 Schlessinger D. Edited by Washington: American Society for Microbiology;
    [Google Scholar]
  10. Ehrlich S.D. 1978; DNA cloning in Bacillus subtilis. . Proceedings of the National Academy of Sciences of the United States of America 75:1433–1436
    [Google Scholar]
  11. Finkelstein M., Rownd R.H. 1978; A rapid method for extracting DNA from agarose gels. Plasmid 1:557–562
    [Google Scholar]
  12. Hansen J.B., Olsen R.H. 1978; Isolation of large bacterial plasmids and characterisation of the P2 incompatibility group plasmids pMGl and pMG5. Journal of Bacteriology 135:227–238
    [Google Scholar]
  13. Humphreys G.O., Willshaw G.A., Anderson E.S. 1975; A simple method for the preparation of large quantities of pure plasmid DNA. Biochimica et biophysica acta 383:457–463
    [Google Scholar]
  14. Ionesco H., Bouanchaud D.H. 1973; Produc-tion de bacteriocine liée a la présence d’un plasmide chez Clostridium perfringens. . Comptes rendus heb-domadaires des seances de l’Academie des sciences D276:2855–2857
    [Google Scholar]
  15. Ionesco H., Bieth G., Dauguet C., Bouanchaud D.H. 1976; Isolement et identifica-tion de deux plasmides d’une souche bacteriocino-gene de Clostridium perfringens. Annales de Micro-biologie 127B:283–294
    [Google Scholar]
  16. Macrina F.L., Kopecko D.J., Jones K.R., Ayres D.J., Mccowen S.M. 1978; A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1:417–420
    [Google Scholar]
  17. Mihelc V.A., Duncan C.L., Chambliss G.H. 1978; Characterisation of a bacteriocinogenic plasmid in Clostridium perfringens. Antimicrobial Agents and Chemotherapy 14:771–779
    [Google Scholar]
  18. Murray K., Murray N.E. 1975; Phage lambda receptor chromosomes for DNA fragments made with restriction endonuclease III of Haemophilus influenzae and restriction endonuclease I of Escherichia coli. Journal of Molecular Biology 98:551–564
    [Google Scholar]
  19. Robson R.L., Robson R.M., Morris J.G. 1974; The biosynthesis of granulose by Clostridium pasteurianum. Biochemical Journal 144:503–511
    [Google Scholar]
  20. Rokos E.A., Rood J.I., Duncan C.L. 1978; Multiple plasmids in different toxigenic types of Clostridium perfringens. . FEMS Microbiology Letters 4:323–326
    [Google Scholar]
  21. Rood J.I., Mahler E.A., Somers E.B., Campos E., Duncan C.L. 1978a; Isolation and characterisation of multiply antibiotic resistant Clostridium perfringens strains from porcine faeces. Antimicrobial Agents and Chemotherapy 13:871–880
    [Google Scholar]
  22. Rood J.I., Scott V.N., Duncan C.L. 1978b; Identification of a transferable tetracycline resistance plasmid (pCW3) from Clostridium perfringens. . Plasmid 1:563–570
    [Google Scholar]
  23. Sarathchandra S.U., Barker A.N., Wolf J. 1974; Studies on the germination of Clostridium butyricum. In Spore Research 1973 pp. 207–231 Barker A.N., Gould G.W., London J.Wolf. Edited by New York: Academic Press.;
    [Google Scholar]
  24. Scott V.N., Duncan C.L. 1978; Cryptic plasmids in Clostridium botulinum and Clostridium botulinumAike organisms. FEMS Microbiology Letters 4:55–58
    [Google Scholar]
  25. Sebald M., Brefort G. 1975; Transfert du plasmide tetracycline-chloramphénicol chez Clostridium perfringens. Comptes rendus hebdomadaires des séances de l’Academie des sciences D281:317–319
    [Google Scholar]
  26. Thomas M., Davis R.W. 1975; Studies on the cleavage of bacteriophage lambda DNA with iscoRI restriction endonuclease. Journal of Molecular Biology 91:315–328
    [Google Scholar]
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