A Gel-stabilized Model Ecosystem for Investigating Microbial Growth in Spatially Ordered Solute Gradients Free

Abstract

The structured nature of microbial ecosystems makes their study difficult, and simple laboratory analogues are needed. Two gel-stabilized gradient systems are described in which solute transfer is by diffusion alone. In the first, organisms grow in a semi-solid agar gel located above a source layer of full-strength agar containing the diffusible solute, which was glucose in the experiments reported here. Changes in physicochemical parameters, various solutes and cell concentration have been monitored in cultures of NCTC 10342 and NCIB 4037 grown in this system. In experiments with a range of bacteria, banded growth was noted for several strains. The microbiology of the water at the base of an oil storage tank was investigated in the second model, in which gas oil was poured over a semi-solid layer containing agar, a basal salts medium, cells and a steel plate. After incubation for up to 90 d the system had differentiated into aerobic and anaerobic regions, and activities included hydrocarbon catabolism, oxygen removal, sulphate reduction, and the growth of a large population of aerobic and anaerobic heterotrophs. The value of these models is discussed with reference to microbial ecology.

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1981-12-01
2024-03-29
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References

  1. Armstrong W. 1975; Waterlogged soils. In Environmental and Plant Ecology pp. 181–218 Etherington J.R. Edited by London:: Wiley;
    [Google Scholar]
  2. Atkinson B., Daoud I.S. 1975; Microbial floes and flocculation in fermentation process engineering. Advances in Biological Engineering 4:41–124
    [Google Scholar]
  3. Atkinson B., Fowler H.W. 1974; The significance of microbial film in fermenters. Advances in Biological Engineering 3:221–277
    [Google Scholar]
  4. Bailey N.T.J. 1959 Statistical Methods in Biology. London:: English University Press.;
    [Google Scholar]
  5. Bazin M.J., Saunders P.T., Prosser J.I. 1976; Models of microbial interaction in the soil. CRC Critical Reviews in Microbiology 4:463–498
    [Google Scholar]
  6. Caldwell D.E., Hirsch P. 1973; Growth of microorganisms in two-dimensional steady-state diffusion gradients. Canadian Journal of Microbiology 19:53–58
    [Google Scholar]
  7. Chebotarev E.N., Gorlenko V.M., Kachalkin V.I. 1974; Microbiological hydrogen sulphide formation in Lake Repnoe (Slavyansk Lakes). Microbiology (English translation of Mikrobiologiya) 42:475–479
    [Google Scholar]
  8. Cooper D.C., Copeland B.J. 1973; Responses of a continuous series of estuarine microecosystems to point-source input variations. Ecological Monographs 43:213–236
    [Google Scholar]
  9. Crowle A.J. 1973 Immunodiffusion, 2nd edn.. London:: Academic Press.;
    [Google Scholar]
  10. Deevey E.S. 1951; Life in the depths of a pond. Scientific American 185:68–72
    [Google Scholar]
  11. Department Of The Environment 1969 Analysis of Raw, Potable and Waste Water. London:: H.M.S.O.;
    [Google Scholar]
  12. Ellwood D.C., Hunter J.R. 1976; The mouth as a chemostat. In Continuous Culture 6: Application and New Fields pp. 270–272 Dean A.C.R., Ellwood D.C., Evans C.G.T., Melling J. Edited by Chichester, England:: Ellis Harwood.;
    [Google Scholar]
  13. Gilchrist J.E., Campbell J.E., Donnelly C.B., Deeler J.T., Delany J.M. 1973; Spiral plate method for bacterial determination. Applied Microbiology 25:244–252
    [Google Scholar]
  14. Greenwood D.J. 1971; Studies on the distribution of oxygen around mustard seedlings (Sinapis albaL.). New Phylologist 70:97–101
    [Google Scholar]
  15. Harvey G.W. 1966; Microlayer collection from the sea surface: a new method and initial results. Limnology and Oceanography 11:608–613
    [Google Scholar]
  16. Henry R.J. 1968 Clinical Chemistry - Principles and Technics pp. 664–666 New York:: Harper & Row.;
    [Google Scholar]
  17. Jarvis B., Lach V.H., Wood J.M. 1977; Evaluation of the spiral plate maker for the enumeration of micro-organisms in foods. Journal of Applied Bacteriology 43:149–157
    [Google Scholar]
  18. Johnson C.M., Nishita M. 1952; Microestimation of sulphur in plant materials, soils and irrigation waters. Analytical Chemistry 24:736–742
    [Google Scholar]
  19. JØrgensen B.B., Revsbech N.P., Blackburn T.H., Cohen Y. 1979; Diurnal cycle of oxygen and sulphidemicrogradients and microbial photo-synthesis in a cyanobacterial mat sediment. Applied and Environmental Microbiology 38:46–58
    [Google Scholar]
  20. Liesegang R.E. 1896; Ueber einige Eigenschaften von Gallerten. Naturwissenschaftliche Wochenschrift 11:353–362
    [Google Scholar]
  21. Lovrrr R.W., Wimpenny J.W.T. 1979; The gradostat: a tool for investigating microbial growth and interactions in solute gradients. Society for General Microbiology Quarterly 6:80
    [Google Scholar]
  22. Lovitt R.W., Wimpenny J.W.T. 1981; The gradostat: a bidirectional compound chemostat and its application in microbiological research. Journal of General Microbiology 127:261–268
    [Google Scholar]
  23. Lynch J.M. 1978; Microbial interaction around imbibed seeds. Annals of Applied Biology 89:165–167
    [Google Scholar]
  24. Marshall K.C. 1976 Interfaces in Microbial Ecology. London:: Harvard University Press.;
    [Google Scholar]
  25. Mortimer C.H. 1942; The exchange of dissolved substances between mud and water in lakes. Journal of Ecology 30:147–201
    [Google Scholar]
  26. Newman H.N., Poole D.F.G. 1974; Structural and ecological aspects of dental plaque. In The Natural Microbial Flora of Man pp. 111–134 Skinner F.A., Carr J.G. Edited by London:: Academic Press.;
    [Google Scholar]
  27. Perfil’ev B.V., Gabe D.R. 1969 Capillary Methods of Investigating Micro-organisms (English translation) Edinburgh:: Oliver & Boyd.;
    [Google Scholar]
  28. Pirt S.J. 1967; A kinetic study of the mode of growth of surface colonies of bacteria and fungi. Journal of General Microbiology 47:181–197
    [Google Scholar]
  29. Raabo E., Terkildsen T.C. 1960; On the enzymatic determination of blood glucose. Scandinavian Journal of Clinical and Laboratory Investigation 12:402–407
    [Google Scholar]
  30. Romanko V.I., Pubienes M.A., Daukshta A.S. 1978; Growth and activity of bacteria on the surface film of water under experimental conditions. Microbiology (English translation of Mikrobiologiya) 47:119–125
    [Google Scholar]
  31. Ruinen J. 1961; The phyllosphere. I. An ecologically neglected milieu. Plant and Soil 15:81–109
    [Google Scholar]
  32. Sorokin Y.I. 1968; Primary production and microbiological processes in Lake Gek Gel. Microbiology (English translation of Mikrobiologiya) 37:289–296
    [Google Scholar]
  33. Spray R.S. 1936; Semisolid media for cultivation and identification of the sporulating anaerobes. Journal of Bacteriology 32:135–155
    [Google Scholar]
  34. Stanley S.O., Malcolm S. 1980; The sediment environment. Society for General Microbiology Quarterly 7:64
    [Google Scholar]
  35. Tschapek M., Gambiagi N. 1954; Die Bildung von Liesegang’schen Ringen durch Azotobacter bei O2-hemmung. Kolloidzeitschrift 135:47–48
    [Google Scholar]
  36. Whittenbury R. 1963; The use of soft agar in the study of conditions affecting the utilization of fermentable substrates by lactic acid bacteria. Journal of General Microbiology 32:375–384
    [Google Scholar]
  37. Williams J.W. 1938a; Bacterial growth “spectrum” analysis. I. Methods and application. American Journal of Medical Technology 4:58–61
    [Google Scholar]
  38. Williams J.W. 1938b; Bacterial growth “spectrum” analysis. II. Their significance in pathology and bacteriology. American Journal of Pathology 14:642–645
    [Google Scholar]
  39. Williams J.W. 1939a; The nature of gel mediums as determined by various gas tensions and its importance in growth of microorganisms and cel-lular metabolism. Growth 3:181–196
    [Google Scholar]
  40. Williams J.W. 1939b; Growth of microorganisms in shake cultures under increased oxygen and carbon dioxide tensions. Growth 3:21–33
    [Google Scholar]
  41. Wimpenny J.W.T. 1979; The growth and form of bacterial colonies. Journal of General Microbiology 114:483–486
    [Google Scholar]
  42. Wimpenny J.W.T., Lewis M.W.A. 1977; The growth and respiration of bacterial colonies. Journal of General Microbiology 103:9–18
    [Google Scholar]
  43. Winogradsky S. 1949 Microbiologie du Sol. Paris:: Masson;
    [Google Scholar]
  44. Zobell C.E. 1941; Studies on marine bacteria. I. Cultural requirements for heterotrophic aerobes. Journal of Marine Research 4:42–47
    [Google Scholar]
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