1887

Abstract

A multistage continuous culture system is described in which solutes are transferred between vessels in opposite directions simultaneously. The system, called a gradostat, produces opposing solute gradients and is a good laboratory model of many natural microbial ecosystems in which solute gradients are important. Theoretical predictions concerning solute transfer were confirmed under steady-state and non steady-state conditions, using a coloured dye. grew anaerobically in the gradostat at the intersection between opposing gradients of succinate and nitrate. Opposing gradients of glucose and oxygen separated the growth of a sp. (a facultative anaerobe) and (an obligate anaerobe). Viable counts for both species fell exponentially away from their growth positions at the ends of the gradostat. The potential value of the gradostat and possible alternative conformations are discussed.

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/content/journal/micro/10.1099/00221287-127-2-261
1981-12-01
2021-08-02
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References

  1. Abson J.W., Todhunter R.K. 1961; Plant for continuous biological treatment of carbonisation effluents. Society of Chemical Industry Monograph 12:147
    [Google Scholar]
  2. American Publich Health Association 1971 Standard Methods for the Examination of Water and Waste Water, 13th edn.. pp. 461–464
    [Google Scholar]
  3. Bazin M.J., Saunders P.T. 1973; Dynamics of nitrification in a continuous flow system. Soil Biology and Biochemistry 5:531–543
    [Google Scholar]
  4. Caldwell D.W., Hirsch P. 1973; Growth of microorganisms in two-dimensional steady state diffusion gradients. Canadian Journal of Microbiology 19:53–58
    [Google Scholar]
  5. Cooper D.G., Copeland B.J. 1973; Responses of a continuous series of estuarine microecosystems to point-source input variations. Ecological Monographs 43:213–236
    [Google Scholar]
  6. Hawkes H.A. 1977; Eutrophication of rivers, effects, causes and control. In Treatment of Industrial Effluents pp. 159–192 Callely A.G., Forster C.F., Stafford D.A. Edited by London: Hodder & Stoughton;
    [Google Scholar]
  7. Herbert D., Phipps P.J., Strange R.E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  8. Holdeman L.V., Cato E.P., Moore W.E.C. 1972 V.P.I. Anaerobe Laboratory Handbook. Blacksburg, Virginia: Virginia Polytechnic Institute and State University;
    [Google Scholar]
  9. Jannasch H.W., Mateles R.I. 1974; Experimental bacterial ecology studied in continuous culture. Advances in Microbial Physiology 11:161–209
    [Google Scholar]
  10. Lloyd D., Brookman J.S.G. 1967; An oxygen electrode reaction vessel. Biotechnology and Bio-engineering 9:271–272
    [Google Scholar]
  11. Lovitt R.W., Wimpenny J.W.T. 1979; The gradostat: a tool for investigating microbial growth and interactions in solute gradients. Society for General Microbiology Quarterly 6:80
    [Google Scholar]
  12. Mackereth F.J.H., Heron J., Talling J.F. 1978; Water Analysis. Freshwater Biological Associtation Scientific Publication36
    [Google Scholar]
  13. Postgate J.R. 1969; Viable counts and viability. Methods in Microbiology 1:611–628
    [Google Scholar]
  14. Pritchard P.M., Ventullo R.M., Suflita J.M. 1976; The microbial degradation of diesel oil in a multistage continuous culture system. In Proceedings of the 3rd International Biodegradation Symposium pp. 67–78 Miles- Sharppey J., Kaplan A.M. Edited by London: Applied Science Publishers;
    [Google Scholar]
  15. Raabo E., Terkildsen T.C. 1960; On the enzymatic determination of blood glucose. Scandinavian Journal of Clinical and Laboratory Investigation 12:402–407
    [Google Scholar]
  16. Wimpenny J.W.T., Coombs J.P., Lovitt R.W., Whittaker S.G. 1981; A gel-stabilized model for investigating microbial growth in spatially ordered solute gradients. Journal of General Microbiology 127:277–287
    [Google Scholar]
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