Germination and Resistance Defects in Spores of a Mutant Lacking a Coat Polypeptide Free

Abstract

Spores produced by a mutant of germinate slowly and are sensitive to lysozyme. The spore coat of the mutant appears to lack a single polypeptide (mol. wt 36000) which normally is deposited in the outer layers of the spore during stage V of sporulation. The mutation maps between and on the chromosome and is genotypically and phenotypically distinct from another mutation which maps in the same region: consequently it has been assigned a new locus The phenotype of the mutant confirms a previous suggestion that lysozyme resistance is conferred by the outermost layers of the spore coat.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-1-81
1981-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/1/mic-127-1-81.html?itemId=/content/journal/micro/10.1099/00221287-127-1-81&mimeType=html&fmt=ahah

References

  1. Akrigg A. 1978; Purification and properties of a manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis. Biochemical Journal 172:69–76
    [Google Scholar]
  2. Aronson A. I., Fitz-James P. C. 1975; Properties of Bacillus cereus spore coat mutants. Journal of Bacteriology 123:354–365
    [Google Scholar]
  3. Ayad S. R., Barker G. R. 1969; The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis. Biochemical Journal 113:167–174
    [Google Scholar]
  4. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  5. Coote J. G. 1972a; Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. Journal of General Microbiology 71:1–15
    [Google Scholar]
  6. Coote J. G. 1972b; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  7. Dancer B. N., Mandelstam J. 1975; Production and possible function of serine protease during sporulation of Bacillus subtilis. Journal of Bacteriology 121:406–410
    [Google Scholar]
  8. Dion P., Mandelstam J. 1980; Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation. Journal of Bacteriology 141:786–792
    [Google Scholar]
  9. Glenn A. R., Mandelstam J. 1971; Sporulation in Bacillus subtilis 168. Comparison of alkaline phosphatase from sporulating and vegetative cells. Biochemical Journal 123:129–138
    [Google Scholar]
  10. Henner D. J., Hoch J. A. 1980; The Bacillus subtilis chromosome. Microbiological Reviews 44:57–82
    [Google Scholar]
  11. Hranueli D., Piggot P. J., Mandelstam J. 1974; Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. Journal of Bacteriology 119:684–690
    [Google Scholar]
  12. Janssen F. W., Lund A. J., Anderson L. E. 1958; Colorimetric assay for dipicolinic acid in bacterial spores. Science 127:26–27
    [Google Scholar]
  13. Jenkinson H. F., Kay D., Mandelstam J. 1980; Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. Journal of Bacteriology 141:793–805
    [Google Scholar]
  14. Jenkinson H. F., Sawyer W. D., Mandelstam J. 1981; Synthesis and order of assembly of spore coat proteins in Bacillus subtilis. Journal of General Microbiology 123:1–16
    [Google Scholar]
  15. Karamata D., Gross J. D. 1970; Isolation and genetic analysis of temperature-sensitive mutants of B. subtilis defective in DNA synthesis. Molecular and General Genetics 108:277–287
    [Google Scholar]
  16. Lacks S., Hotchkiss R. D. 1960; A study of the genetic material determining an enzyme activity in Pneumococcus. Biochimica et biophysica acta 39:508–518
    [Google Scholar]
  17. Laemmli U. K., Favre M. 1973; Maturation of the head of bacteriophage T4. I. DNA packaging events. Journal of Molecular Biology 80:575–599
    [Google Scholar]
  18. Lafferty E., Moir A. 1977; Further studies on conditional germination mutants of Bacillus subtilis 168. In Spore Research 1976 pp. 87–105 Barker A. N., Wolf J., Ellar D. J., Dring G. J., Gould G. W. Edited by London: Academic Press.;
    [Google Scholar]
  19. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  21. Moir A., Lafferty E., Smith D. A. 1979; Genetic analysis of spore germination mutants of Bacillus subtilis 168: the correlation of phenotype with map location. Journal of General Microbiology 111:165–180
    [Google Scholar]
  22. Pandey N. K., Aronson A. I. 1979; Properties of the Bacillus subtilis spore coat. Journal of Bacteriology 137:1208–1218
    [Google Scholar]
  23. Piggot P. J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  24. Piggot P. J., Coote J. G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  25. Sadoff H. L. 1966; Glucose dehydrogenase - soluble. I. Bacillus cereus. Methods in Enzymology 9:103–107
    [Google Scholar]
  26. Stelma G. N. Jr Aronson A. I., Fitz-James P. 1978; Properties of Bacillus cereus temperature- sensitive mutants altered in spore coat formation. Journal of Bacteriology 134:1157–1170
    [Google Scholar]
  27. Stelma G. N. Jr Aronson A. I., Fitz-James P. C. 1980; A Bacillus cereus mutant defective in spore coat deposition. Journal of General Microbiology 116:173–185
    [Google Scholar]
  28. Sterlini J. M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  29. Takahashi I. 1963; Transducing phages for Bacillus subtilis. Journal of General Microbiology 31:211–217
    [Google Scholar]
  30. Trowsdale J., Smith D. A. 1975; Isolation, characterization, and mapping of Bacillus subtilis 168 germination mutants. Journal of Bacteriology 123:83–95
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-1-81
Loading
/content/journal/micro/10.1099/00221287-127-1-81
Loading

Data & Media loading...

Most cited Most Cited RSS feed