Spores produced by a mutant of germinate slowly and are sensitive to lysozyme. The spore coat of the mutant appears to lack a single polypeptide (mol. wt 36000) which normally is deposited in the outer layers of the spore during stage V of sporulation. The mutation maps between and on the chromosome and is genotypically and phenotypically distinct from another mutation which maps in the same region: consequently it has been assigned a new locus The phenotype of the mutant confirms a previous suggestion that lysozyme resistance is conferred by the outermost layers of the spore coat.


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