1887

Abstract

The specific activities of the key enzymes involved in the biosynthesis of the exopolysaccharide alginic acid by were determined in extracts of batchcultured organisms grown with different carbon sources in the presence of limited and excess inorganic phosphate. Alginic acid production was also measured. Glucose, fructose, sorbitol, mannitol, glycerol and gluconate resembled sucrose in supporting much greater alginate production in media containing growth-limiting amounts of inorganic phosphate. Mannose supported only poor growth with no alginate formation, and growth did not occur on acetate. Increases in the specific activities of phosphomannose isomerase, GDPmannose pyrophosphorylase and GDPmannose dehydrogenase were accompanied by increased alginic acid production. Our results accord with the suggestion that alginate formation is controlled by derepression of key biosynthetic enzymes.

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1981-11-01
2021-05-08
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References

  1. Anderson R. L., Sapico V. L. 1975; d-Fructose (d-mannose) kinase. Methods in Enzymology 42:39–43
    [Google Scholar]
  2. Carter I. S., Dawes E. A. 1979; Effect of oxygen concentration and growth rate on glucose metabolism, poly-β-hydroxybutyrate biosynthesis and respiration of Azotobacter beijerinckii. Journal of General Microbiology 110:393–400
    [Google Scholar]
  3. Clarke D. J., Morris J. G. 1976; Partial purification of a dicyclohexylcarbodiimide-sensitive membrane adenosine triphosphatase complex from the obligately anaerobic bacterium Clostridium pasteurianum. Biochemical Journal 154:725–729
    [Google Scholar]
  4. Couperwhite I., Mccallum M. F. 1975; Polysaccharide production and the possible occurrence of GDP-d-mannose dehydrogenase in Azotobacter vinelandii. Antonie van Leeuwenhoek 48:25–32
    [Google Scholar]
  5. Dawes E. A., Senior P. J. 1973; The role and regulation of energy reserve polymers in microorganisms. Advances in Microbial Physiology 10:135–266
    [Google Scholar]
  6. Deavin L. 1976 The production of polysaccharide by Azotobacter vinelandii. Ph.D. thesis Brunel University, U.K.:
    [Google Scholar]
  7. Deavin L., Jarman T. R., Lawson C. J., Righelato R. C., Slocombe S. 1977; The production of alginic acid by Azotobacter vinelandii in batch and continuous culture. In Extracellular Microbial Polysaccharides pp. 14–26 Sandford P. A., Laskin A. Edited by Washington: American Chemical Society.;
    [Google Scholar]
  8. Doggett R. G., Harrison G. M. 1969; Significance of the pulmonary flow associated with chronic pulmonary disease in cystic fibrosis. Proceedings of the 5th International Cystic Fibrosis Conference pp. 175–188 Lawson D. Edited by London: Cambridge University Press.;
    [Google Scholar]
  9. Doudoroff M. 1962; Mannose isomerase of Pseudomonas saccharophila. Methods in Enzymology 5:335–338
    [Google Scholar]
  10. Gorin P. A. J., Spencer J. F. T. 1966; Exocellularalginic acid from Azotobacter vinelandii. Canadian Journal of Chemistry 44:993–998
    [Google Scholar]
  11. Gracy R. W., Noltmann E. A. 1968; Studies on phosphomannose isomerase. Journal of Biological Chemistry 243:3161–3168
    [Google Scholar]
  12. Grant W. D., Sutherland I. W., Wilkinson J. F. 1970; Control of colanic acid synthesis. Journal of Bacteriology 103:89–96
    [Google Scholar]
  13. Jarman T. R. 1979; Bacterial alginate synthesis. In Microbial Polysaccharides and Polysaccharases pp. 35–50 Berkeley R. C. W., Gooday G. W., Ellwood D. C. Edited by London: Academic Press.;
    [Google Scholar]
  14. Jarman T. R., Deavin L., Slocombe S., Righelato R. C. 1978; Investigation of the effect of environmental conditions on the rate of exopolysaccharide synthesis in Azotobacter vinelandii. Journal of General Microbiology 107:59–64
    [Google Scholar]
  15. Kornfield R. H., Ginsburg V. 1966; Control of synthesis of guanosine 5′-diphosphate d-mannose and guanosine 5′-diphosphate d-fucose in bacteria. Biochimica et biophysica acta 117:79–87
    [Google Scholar]
  16. Law J. H., Slepecky R. A. 1961; Assay of poly-β-hydroxybutyric acid. Journal of Bacteriology 82:33–36
    [Google Scholar]
  17. Markovitz A. 1977; Genetics and regulation of bacterial capsular polysaccharide biosynthesis and radiation sensitivity. In Surface Carbohydrates of the Prokaryotic Cell pp. 415–482 Sutherland I. W. Edited by London: Academic Press.;
    [Google Scholar]
  18. Midgley M., Dawes E. A. 1973; The regulation of transport of glucose and methyl α-glucoside in Pseudomonas aeruginosa. Biochemical Journal 132:141–154
    [Google Scholar]
  19. Munch-Petersen A. 1962; GDPM pyrophos-phorylase. Methods in Enzymology 5:171–174
    [Google Scholar]
  20. Norval M., Sutherland I. W. 1973; The production of enzymes involved in exopolysaccharide synthesis in Klebsiella aerogenes, Types 1 and 8. European Journal of Biochemistry 35:209–215
    [Google Scholar]
  21. Olins A. L., Warner R. C. 1967; Physicochemical studies on a lipopolysaccharide from the cell wall of Azotobacter vinelandii. Journal of Biological Chemistry 242:4994–5001
    [Google Scholar]
  22. Pindar D. F., Bucke C. 1975; The biosynthesis of alginic acid by Azotobacter vinelandii. Biochemical Journal 152:617–622
    [Google Scholar]
  23. Preiss J. 1966; GDP-mannose pyrophosphorylase from Arthrobacter. Methods in Enzymology 8:271–275
    [Google Scholar]
  24. Scott G. 1979 Glycosyl transfer mechanisms in the biosynthesis of alginic acid. Ph.D. thesis University of Nottingham, U.K.:
    [Google Scholar]
  25. Sutherland I. W. 1977; Microbial exopolysaccharide synthesis. In Extracellular Microbial Polysaccharides pp. 40–57 Sandford P. A., Laskin A. Edited by Washington: American Chemical Society.;
    [Google Scholar]
  26. Sutherland I. W. 1979; Microbial exopolysaccharides: control of synthesis and acylation. In Microbial Polysaccharides and Polysaccharases pp. 1–34 Berkeley R. C. W., Gooday G. W., Ellwood D. C. Edited by London: Academic Press.;
    [Google Scholar]
  27. Sweeley C. C., Bentley R., Makita M., Wells W. W. 1963; Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. Journal of the American Chemical Society 85:2497–2507
    [Google Scholar]
  28. Wilkinson S. G. 1977; Composition and structure of bacterial lipopolysaccharides. In Surface Carbohydrates of the Prokaryotic Cell pp. 97–175 Sutherland I. W. Edited by London: Academic Press.;
    [Google Scholar]
  29. Williams A. G., Wimpenny J. W. T. 1980; Extracellular polysaccharide biosynthesis by Pseudomonas NCIB 11264. Studies on precursorforming enzymes and factors affecting exopolysaccharide production by washed suspensions. Journal of General Microbiology 116:133–141
    [Google Scholar]
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