1887

Abstract

A species of the imperfect fungus produced extracellular, intracellular and mycelial-bound -glucosidases when grown on cellulose. The extracellular enzyme was induced on cellulose but was repressed by cellobiose and glucose. Derepression of this enzyme occurred when cellobiose and glucose were nearly exhausted from the growth medium. Intracellular and mycelial-bound -glucosidases were induced on cellulose and cellobiose but appeared to be repressed on glucose and lactose. One extracellular (EG-1) and two intracellular (IG-1, IG-2) -glucosidases were produced when sp. was grown on cellulose. The molecular weights of EG-1 and IG-2 were 37500 and that of IG-1 was 480000. The of EG-1 and IG-2 was 8 × 10 M for -nitrophenyl---glucoside (PNPG), and 5·7 × 10 M for cellobiose; the for glucose, a competitive inhibitor, was 6·7 × 10 . The of IG-1 was 1·67 × 10 for PNPG and 2·0 × 10 for cellobiose; the for glucose, a non-competitive inhibitor, was 1·63 × 10 . -Glucosidases EG-1 and IG-2 showed optimal activity at pH4.5 whilst the pH optimum for IG-1 was 4·2. Temperature optima were 50 °C for EG-1 and IG-2, and 60–65 °C for IG-1. Their respective isoelectric points were 8·3, 8·3 and 4·4.

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1981-11-01
2024-12-05
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References

  1. Andrews P. 1970; Estimation of molecular size and molecular weights of biological compounds by gel filtration. Methods of Biochemical Analysis 18:1–53
    [Google Scholar]
  2. Berg B., Pettersson G. 1977; Location and formation of cellulases in Trichoderma viride. Journal of Applied Bacteriology 42:65–75
    [Google Scholar]
  3. Dekker R. F. H. 1980; Induction and characterization of a cellobiose dehydrogenase produced by a species of Monilia. Journal of General Microbiology 120:309–316
    [Google Scholar]
  4. Dekker R. F. H., Lindner W. A. 1979; Bioutilization of lignocellulosic waste materials: a review. South African Journal of Science 76:65–71
    [Google Scholar]
  5. Dekker R. F. H., Richards G. N. 1971; Determination of starch in plant material. Journal of the Science of Food and Agriculture 22:441–444
    [Google Scholar]
  6. Deshpande V., Eriksson K. E., Pettersson B. 1978; Production, purification and partial characterization of l,4-β-glucosidase enzymes from Sporotrichum pulverulenlum. European Journal of Biochemistry 90:191–198
    [Google Scholar]
  7. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  8. Eberhart B. A., Beck R. S. 1970; Localization of the β-glucosidases in Neurospora crassa. Journal of Bacteriology 101:408–417
    [Google Scholar]
  9. Eberhart B. M., Beck R. S. 1973; Induction of β-glucosidases in Neurospora crassa. Journal of Bacteriology 116:295–303
    [Google Scholar]
  10. Eriksson K. E. 1978; Enzyme mechanisms involved in cellulose hydrolysis by the rot fungus Sporotrichum pulverulenlum. Biotechnology and Bioengineering 20:317–332
    [Google Scholar]
  11. Gong C. S., Ladisch M. R., Tsao G. T. 1977; Cellobiase from Trichoderma viride: purification, properties, kinetics and mechanism. Biotechnology and Bioengineering 19:959–981
    [Google Scholar]
  12. Hägerdal B. G. R., Ferchak J. D., Pye E. K. 1978; Cellulolytic enzyme system of Thermoactinomyces sp. grown on microcrystalline cellulose. Applied and Environmental Microbiology 36:606–612
    [Google Scholar]
  13. Hartree E. F. 1972; Determination of protein: a modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48:422–427
    [Google Scholar]
  14. Herr D., Baumer F., Dellweg H. 1978; Purification and properties of an extracellular β-giucosidase from Lenziles trabea. European Journal of Applied Microbiology and Biotechnology 5:29–36
    [Google Scholar]
  15. Kwang J. T., Suzuki H. 1976; Intracellular distribution of a cellulolytic pseudomonad. Agricultural and Biological Chemistry 40:2169–2175
    [Google Scholar]
  16. Lusis A. J., Becker R. R. 1973; The β-glucosidase system of the thermophilic fungus Chaetomium thermophile var.coprophile n. var. Biochimica et biophysica acta 329:5–16
    [Google Scholar]
  17. Maguire R. J. 1977; Kinetics of the hydrolysis of cellobiose and β-nitrophenyl-β-d-glucoside by cellobiase of Trichoderma viride. Canadian Journal of Microbiology 55:19–26
    [Google Scholar]
  18. Mandels M., Reese E. T. 1957; Induction of cellulase in Trichoderma viride as influenced by carbon sources and metals. Journal of Bacteriology 73:269–278
    [Google Scholar]
  19. O’Day D. H., Paterno G. D. 1979; Intracellular and extracellular CM-cellulase and β-glucosidase activity during germination of Polysphondylium pallidum microcysts. Archives of Microbiology 121:231–234
    [Google Scholar]
  20. Pettersson L. G. 1975; The mechanism of enzymatic hydrolysis of cellulose by Trichoderma viride. In Symposium on Enzymatic Hydrolysis of Cellulose pp. 255–261 Bailey M., Enari T. M., Linko M. Edited by Helsinki,Finland: SITRA.;
    [Google Scholar]
  21. Reese E. T., Maguire A. 1971; Increase in cellulase yields by addition of surfactants to cellobiose cultures of Trichoderma viride. Developments in Industrial Microbiology 12:212–224
    [Google Scholar]
  22. Sandermann H., Strominger J. L. 1972; Purification and properties of C55-isoprenoid alcohol kinase from Staphylococcus aureus. Journal of Biological Chemistry 247:5123–5131
    [Google Scholar]
  23. Smith M. H., Gold M. H. 1979; Phanerochaete chrysosporium β-glucosidases: induction, cellular localization and physical characterization. Applied and Environmental Microbiology 37:938–942
    [Google Scholar]
  24. Sternberg D. 1976; β-Glucosidases of Trichoderma: its biosynthesis and role in saccharification of cellulose. Applied and Environmental Microbiology 31:648–654
    [Google Scholar]
  25. Walseth C. S. 1952; Occurrence of cellulases in enzyme preparations from microorganisms. Technical and Paper Pulp Institute (TAPPI) 35:228–233
    [Google Scholar]
  26. Wilson R. W., Niederpruem D. J. 1967; Control of β-glucosidases in Schizophyllum commune. Canadian Journal of Microbiology 13:1009–1020
    [Google Scholar]
  27. Wood T. M., Mccrae S. I. 1977; The mechanism of cellulase action with particular reference to the C1 component. In Bioconversion of Cellulosic Substances into Energy, Chemicals and Microbial Protein pp. 111–141 Ghose T. K. Delhi, India: IIT.;
    [Google Scholar]
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