1887

Abstract

In studies aimed at identifying the determinants responsible for the ability of gonococci to survive and grow within human phagocytes, the reduction of intracellular survival of phagocyte-resistant gonococci by prior treatment of the bacteria with homologous antiserum provided two indirect means of testing for possible determinant). First, surface washes of the resistant gonococci and fractions of these extracts were examined for ability to neutralize the above effect of antisera. Second, antisera raised to purified surface components of the resistant organisms were examined for ability to promote intracellular killing. A combination of these methods indicated that the aggressins were not pili but components of the outer membrane.

A surface wash of a phagocyte-resistant, pilated strain, BS4 (agar), neutralized the activity of homologous antisera before and after centrifuging at 20000 , but pili separated from the supernatant did not. A corresponding supernatant from the surface wash of a phagocyte-susceptible strain, BSSH, had little neutralizing ability. Antisera to pili failed to reduce intracellular survival of resistant gonococci, whereas antisera against outer membrane vesicles did so. Finally, the neutralizing activity of the surface wash supernatant was removed by centrifugation after treatment with wheat germ agglutinin, which precipitates outer membrane vesicles.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-1-103
1981-11-01
2021-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/1/mic-127-1-103.html?itemId=/content/journal/micro/10.1099/00221287-127-1-103&mimeType=html&fmt=ahah

References

  1. Bonting S. L., Jones M. 1957; Determination of microgram quantities of deoxyribonucleic acid and protein in tissues grown in vitro. Archives of Biochemistry and Biophysics 66:340–353
    [Google Scholar]
  2. Casey S. G., Veale D. R., Smith H. 1979; Demonstration of intracellular growth of gonococci in human phagocytes using spectinomycin to kill extracellular organisms. Journal of General Microbiology 113:395–398
    [Google Scholar]
  3. Casey S. G., Veale D. R., Smith H. 1980; Intracellular survival of Neisseria gonorrhoeae in human urethral exudate. FEMS Microbiology Letters 8:97–100
    [Google Scholar]
  4. Farzadegan H., Roth I. L. 1975; Scanning electron-microscopy and freeze-etching of gonorrhoeal urethral exudate. British Journal of Venereal Disease 51:83–91
    [Google Scholar]
  5. Heckels J. E. 1977; The surface properties of Neisseria gonorrhoeae, isolation of the major components of the outer membrane. Journal of General Microbiology 99:333–341
    [Google Scholar]
  6. Johnston K. H. 1978 In Immunobiology of Neisseria gonorrhoeae pp. 121–129 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  7. Lambden P. R., Heckels J. E. 1979; The influence of outer-membrane protein composition on the colonial morphology of Neisseria gonorrhoeae. FEMS Microbiology Letters 5:263–265
    [Google Scholar]
  8. Novotny P., Short J. A., Walker P. D. 1975; An electron-microscopic study of naturally occurring and cultured cells of Neisseria gonorrhoeae. Journal of Medical Microbiology 8:413–427
    [Google Scholar]
  9. Ovchinnikov N. M., Delektorskij V. V. 1977; Phagocytosis in urethral discharge from patients with gonorrhoea. In Gonorrhoea: Epidemiology and Pathogenesis pp. 157–183 Skinner J. A., Walker P. D., Smith H. Edited by London: Academic Press.;
    [Google Scholar]
  10. Parsons N. J., Penn C. W., Veale D. R., Smith H. 1979; More than one antigen contributes to the immunogenicity of Neisseria gonorrhoeae in the guinea-pig subcutaneous chamber model. Journal of General Microbiology 113:97–104
    [Google Scholar]
  11. Parsons N. J., Penn C. W., Veale D. R., Smith H. 1980; The complexity of immunogenicity of Neisseria gonorrhoeae in the guinea pig subcutaneous chamber model. Journal of General Microbiology 118:523–527
    [Google Scholar]
  12. Penn C. W., Sen D., Veale D. R., Witt K., Parsons N. J., Smith H. 1976; Morphological, biological and antigenic properties of Neisseria gonorrhoeae adapted to growth in guinea-pig subcutaneous chambers. Journal of General Microbiology 97:35–43
    [Google Scholar]
  13. Penn C. W., Veale D. R., Smith H. 1977; Selection from gonococci grown in vitro of a colony type with some virulence properties of organisms adapted in vivo. Journal of General Microbiology 100:147–158
    [Google Scholar]
  14. Penn C. W., Parsons N. J., Veale D. R., Smith H. 1978; Correlation with different immunotypes of gonococcal antigens associated with growth in vivo. Journal of General Microbiology 105:153–157
    [Google Scholar]
  15. Penn C. W., Parsons N. J., Veale D. R., Smith H. 1980; Antigenic heterogeneity associated with pilus aggregation and autoagglutinability in Neisseria gonorrhoeae. Journal of General Microbiology 121:195–202
    [Google Scholar]
  16. Perera V. Y., Penn C. W., Smith H. 1980; The use of specific antiserum induced by lectin-antigen complexes to investigate the outer membrane antigens of Neisseria gonorrhoeae. Journal of Immunological Methods 37:175–184
    [Google Scholar]
  17. Robertson J. N., Vincent P., Ward M. E. 1977; The preparation and properties of gonococcal pili. Journal of General Microbiology 102:169–177
    [Google Scholar]
  18. Smith H. 1980; Introduction. In The Molecular Basis of Microbial Pathogenicity, Dahlem Konferenzen 1980 pp. 11–16 Smith H., Skehel J. J., Turner M. J. Edited by Weinheim: Verlag Chemie.;
    [Google Scholar]
  19. Sparling P. F. 1979; Use of microbial genetics in the study of pathogenicity; differentiation between correlation and causation. In Microbiology 1979 pp. 249–253 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  20. Swanson J. 1978; Cell wall outer membrane variants of Neisseria gonorrhoeae. In Immunobiology of Neisseria gonorrhoeae pp. 130–137 Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Edited by Washington, D.C.: American Society for Microbiology.;
    [Google Scholar]
  21. Veale D. R., Smith H., Witt K., Marshall R. B. 1975; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in subcutaneous perforated plastic chambers in guinea-pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  22. Veale D. R., Finch H., Smith H., Witt K. 1976; Penetration of penicillin into human phagocytes containing Neisseria gonorrhoeae: intracellular survival and growth at optimum concentrations of antibiotic. Journal of General Microbiology 95:353–363
    [Google Scholar]
  23. Veale D. R., Penn C. W., Sutton S., Smith H. 1978; The effects of specific antiserum on the resistance of Neisseria gonorrhoeae to intracellular killing by phagocytes of human blood. Journal of General Microbiology 106:129–136
    [Google Scholar]
  24. Veale D. R., Goldner M., Penn C. W., Ward J., Smith H. 1979; The intracellular survival and growth of gonococci in human phagocytes. Journal of General Microbiology 113:383–393
    [Google Scholar]
  25. Ward M. E., Glynn A. A., Watt P. J. 1972; The fate of gonococci in polymorphonuclear leukocytes: an electron-microscopic study of the natural disease. British Journal of Experimental Pathology 53:289–294
    [Google Scholar]
  26. Witt K., Veale D. R., Finch H., Penn C. W., Sen D., Smith H. 1976a; Resistance of Neisseria gonorrhoeae grown in vivo to ingestion and digestion by phagocytes of human buffy coat. Journal of General Microbiology 96:341–350
    [Google Scholar]
  27. Witt K., Veale D. R., Smith H. 1976b; Resistance to ingestion and digestion of Neisseria gonorrhoeae by phagocytes of human buffy coat. Journal of Medical Microbiology 9:1–12
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-1-103
Loading
/content/journal/micro/10.1099/00221287-127-1-103
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error