RT Journal Article SR Electronic(1) A1 Archer, David B. A1 Barber, ChristineYR 1981 T1 Isolation and Restriction Mapping of a Spiroplasma Plasmid JF Microbiology, VO 126 IS 2 SP 511 OP 514 DO https://doi.org/10.1099/00221287-126-2-511 PB Microbiology Society, SN 1465-2080, AB A plasmid, pIJ2000, of approximately 7#x00B7;8 kilobase pairs (kb) and with a base composition of 22ยท4 mol% (G + C) has been isolated from the non-helical Spiroplasma citri strain ASP-1. pIJ2000 was the only covalently-closed circular DNA molecule detected in cleared ly sates of high passage cultures of strain ASP-1 following CsCl/ethidium bromide equilibrium buoyant density centrifugation. A primary isolate of strain ASP- 1 contained two additional plasmids of approximately 35#x00B7;4 kb and 14#x00B7;8 kb. pIJ2000 contained single sites for the restriction endonucleases BgZlII, HaeIII, HindIII and XhoI arid three sites for MboI., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-126-2-511