1887

Abstract

A plasmid, pIJ2000, of approximately 7#x00B7;8 kilobase pairs (kb) and with a base composition of 22·4 mol% (G + C) has been isolated from the non-helical Spiroplasma citri strain ASP-1. pIJ2000 was the only covalently-closed circular DNA molecule detected in cleared ly sates of high passage cultures of strain ASP-1 following CsCl/ethidium bromide equilibrium buoyant density centrifugation. A primary isolate of strain ASP- 1 contained two additional plasmids of approximately 35#x00B7;4 kb and 14#x00B7;8 kb. pIJ2000 contained single sites for the restriction endonucleases II, III, III and I arid three sites for I.

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/content/journal/micro/10.1099/00221287-126-2-511
1981-10-01
2021-05-15
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