A plasmid, pIJ2000, of approximately 7·8; kilobase pairs (kb) and with a base composition of 22·4; mol % (G + C) has been isolated from the non-helical strain ASP-1. pIJ2000 was the only covalently-closed circular DNA molecule detected in cleared lysates of high passage cultures of strain ASP-1 following CsC1/ethidium bromide equilibrium buoyant density centrifugation. A primary isolate of strain ASP-1 contained two additional plasmids of approximately 35·4; kb and 14·8; kb. pIJ2000 contained single sites for the restriction endonucleases /II, III, dIII and I and three sites for I.


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