1887

Abstract

fusion strains of K12 have been constructed using the Casadaban (1976) gene-fusion technique. The major modification to the procedure was the removal of the guanine requirement of Mu-induced auxotrophs by introduction of a ColEl- plasmid prior to selection of the fusion. Conversion to prototrophy was necessary to overcome problems associated with repression of the operon by guanine added to selection media. Induction of the lysogenic fusion strains yielded plaque-forming transducing phages that carried the genes and either the complete gene and the promoter or only a distal portion of .

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/content/journal/micro/10.1099/00221287-126-2-497
1981-10-01
2021-08-04
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