fusion strains of K12 have been constructed using the Casadaban (1976) gene-fusion technique. The major modification to the procedure was the removal of the guanine requirement of Mu-induced auxotrophs by introduction of a ColEl- plasmid prior to selection of the fusion. Conversion to prototrophy was necessary to overcome problems associated with repression of the operon by guanine added to selection media. Induction of the lysogenic fusion strains yielded plaque-forming λ transducing phages that carried the genes and either the complete gene and the promoter or only a distal portion of .


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error