Glycollate Metabolism in the Obligate Chemolithotroph Grown in Continuous Culture Free

Abstract

CO-limited chemostat cultures of excreted 13·5; nmol glycollate min (mg protein). This confirmed the earlier finding that glycollate excretion by is dependent on the CO/O ratio applied in the chemostat and thus is probably due to the oxygenase activity of -ribulose-1,5-bisphosphate (RuBP) carboxylase. RuBP-dependent oxygen uptake and activity of phosphoglycollate phosphatase were demonstrated in cell-free extracts of .

was able to metabolize exogenous glycollate only to a limited extent. The proportion of dry weight that was derived from [1-C]glycollate by both CO- and thiosulphate-limited chemostat cultures was only 0·7 % of the total. Short-term labelling experiments with [1-C]glycollate and enzyme studies indicated that can metabolize glycollate via two pathways. The enzymes of the glycine-serine pathway were demonstrated in cell-free extracts, but on the basis of kinetic experiments it was concluded that this pathway does not play a major role in glycollate metabolism. Malate was the major primary labelling product. Glyoxylate, formed from glycollate, may be incorporated into malate via malate synthase, which was detected in cell-free extracts of .

The activities of the enzymes needed for the metabolism of glycollate were independent of growth conditions applied in the chemostat. The results are discussed in relation to the possible causes of obligate chemolithotrophy.

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1981-10-01
2024-03-28
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References

  1. Bamforth C. W., Quayle J. R. 1977; Hydroxy- pyruvate reductase activity in Paracoccus denitrificans. Journal of General Microbiology 101:259–267
    [Google Scholar]
  2. Beck E. 1979; Glycolate synthesis. In Photosynthesis II (Encyclopedia of Plant Physiology, New Series 6 pp. 327–335 Pirson A., Zimmerman M. H. Edited by Berlin:: Springer Verlag.;
    [Google Scholar]
  3. Beudeker R. F., Cannon G. C., Kuenen J. G., Shively J. M. 1980; Relations between d-ribulose-l,5-bisphosphate carboxylase, carboxysomes and CO2-fixing capacity in the obligate chemolithotrophThiobacillus neapolitanusgrown under different limitations in the chemostat. Archives of Microbiology 124:185–191
    [Google Scholar]
  4. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Braun W., Kaltwasser H. 1979; Untersuchungen zum Glyoxylsaäurewechsel von Bacillus fastidiosusStamm 83. Archives of Microbiology 121:129–134
    [Google Scholar]
  6. Chandra T. S., Shethna Y. I. 1977; Oxalate, formate, formamide and methanol metabolism in Thiobacillus novellus. Journal of Bacteriology 131:389–398
    [Google Scholar]
  7. Chollet R. 1977; The biochemistry of photo-respiration. Trends in Biochemical Sciences 2:155–159
    [Google Scholar]
  8. Codd G. A., Schmid G. H. 1972; Serological characterization of the glycollate oxidizing enzymes from tobacco, Euglena gracilisand a yellow mutant of Chlorella vulgaris. Plant Physiology 50:769–773
    [Google Scholar]
  9. Codd G. A., Stewart W. D. P. 1973; Pathways of glycollate metabolism in the blue green alga Anabaena cylindrica. Archiv für Mikrobiologie 94:11–28
    [Google Scholar]
  10. Codd G. A., Bowien B., Schlegel H. G. 1976; Glycollate production and excretion by Alcaligenes eutrophus. Archives of Microbiology 110:167–171
    [Google Scholar]
  11. Cohen Y., De Jonge I., Kuenen J. G. 1979; Excretion of glycollate by Thiobacillus neapolitanusin continuous culture. Archives of Microbiology 122:189–194
    [Google Scholar]
  12. Colby J., Dalton H., Whittenbury R. 1979; Biological and biochemical aspects of microbial growth on C, compounds. Annual Review of Microbiology 33:481–517
    [Google Scholar]
  13. Hoban D. J., Lyric R. M. 1975; Phosphoenol-pyruvate carboxylase of Thiobacillus thioparus1.General properties. Canadian Journal of Bio-chemistry 53:875–880
    [Google Scholar]
  14. Jensen R. G., Bahr J. T. 1977; Ribulose-1,5-bisphosphate carboxylase-oxygenase. Annual Review of Plant Physiology 28:379–400
    [Google Scholar]
  15. Johnson E. J., Abraham S. 1969; Enzymes of intermediary carbohydrate metabolism in the obligate autotrophs Thiobacillus thioparusand Thiobacillus neapolitanus. Journal of Bacteriology 100:962–968
    [Google Scholar]
  16. Kelly D. P. 1967; The incorporation of acetate by the chemoautotroph Thiobacillus neapolitanusstrain C. Archiv für Mikrobiologie 58:99–116
    [Google Scholar]
  17. Kelly D. P. 1971; Autotrophy: concepts of lithotrophic bacteria and their organic metabolism. Annual Review of Microbiology 25:177–210
    [Google Scholar]
  18. Kornberg H. L., Morris J. G. 1963; β-Hydroxyaspartate pathway: a new route for biosynthesis from glyoxylate. Nature; London: 197456–457
    [Google Scholar]
  19. Kuenen J. G. 1979; Growth yields and maintenance energy requirement in Thiobacillusspecies under energy limitation. Archives of Microbiology 122:183–188
    [Google Scholar]
  20. Kuenen J. G., Veldkamp H. 1973; Effects of organic compounds on chemostat cultures of Thiomicrospira pelophila, Thiobacillus thioparusand Thiobacillus neapolitanus. Archiv für Mikrobiologie 94:173–190
    [Google Scholar]
  21. Lewis K. F., Weinhouse S. 1957; Determination of glycolic, glyoxylic and oxalic acids. Methods in Enzymology 3:272–273
    [Google Scholar]
  22. Long A. G., Quayle J. R., Stedtman R. J. 1951; The separation of acids by paper partition chromatography. Journal of the Chemical Society2197–2201
    [Google Scholar]
  23. Lorimer G. H., Badger M. R., Andrews T. J. 1977; d-Ribulose-l,5-bisphosphate carboxylase-oxygenase. Analytical Biochemistry 78:66–75
    [Google Scholar]
  24. Matin A. 1978; Organic nutrition of chemolithotrophic bacteria. Annual Review of Microbiology 32:433–469
    [Google Scholar]
  25. Postgate J. 1974; Prerequisites for biological nitrogen fixation in free living heterotrophic bacteria. In The Biology of Nitrogen Fixation pp. 663–687 Quispel A. Edited by Amsterdam:: Elsevier/North Holland.;
    [Google Scholar]
  26. Rittenberg S. C. 1969; The roles of exogenous organic matter in the physiology of chemolithotrophic bacteria. Advances in Microbial Physiology 3:159–196
    [Google Scholar]
  27. Rowell P., Stewart W. D. P. 1976; Alanine dehydrogenase of the N2-fixing blue-green alga Anabaena cylindrica. Archives of Microbiology 107:115–124
    [Google Scholar]
  28. Sahl H. G., Truper H. G. 1977; Enzymes of CO2 fixation in Chromatiaceae. FEMS Microbiology Letters 2:129–132
    [Google Scholar]
  29. Saxena J., Vishniac W. 1970; Influence of organic acids on the growth of Thiobacillus neapolitanus. Antonie van Leeuwenhoek 36:109–118
    [Google Scholar]
  30. SchÜrmann P. 1969; Separation of phosphate esters and algal extracts by thin-layer electrophoresis and chromatography. Journal of Chromatography 39:507–509
    [Google Scholar]
  31. Smith A. J., Hoare D. S. 1977; Specialist phototrophs, lithotrophs and methylotrophs: a unity among a diversity of prokaryotes?. Bacteriological Reviews 41:419–448
    [Google Scholar]
  32. Taylor S. C., Dalton H., Dow S. C. 1981; Ribulose-1,5-bisphosphate carboxylase/oxygenase and carbon assimilation in Methylococcus capsulatus(Bath). Journal of General Microbiology 122:89–94
    [Google Scholar]
  33. Tolbert N. E. 1976; Glycollate oxidase and glycollate dehydrogenase in marine algae and plants. Australian Journal of Plant Physiology 3:129–132
    [Google Scholar]
  34. Tolbert N. E. 1979; Glycollate metabolism by higher plants and algae. In Photosynthesis II (Encyclopedia of Plant Physiology, New Series 6 pp. 338–351 Pirson A., Zimmerman M. H. Edited by Berlin:: Springer Verlag.;
    [Google Scholar]
  35. Vishniac W., Santer M. 1957; The thiobacilli. Bacteriological Reviews 26:168–175
    [Google Scholar]
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