Attempts were made to isolate open and covalently closed circular DNA from strains containing the IncJ plasmids. All of the methods tried were unsuccessful. It was shown that the IncJ plasmid R391 can integrate into the K12 chromosome and can mobilize chromosomal markers from a single origin in an orientated manner. It is proposed that the IncJ plasmids are integrated in the chromosome for most, if not all, of their existence and this explains the inability to isolate plasmid DNA from strains containing them.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error