1887

Abstract

Fungal and bacterial oxidation of steroid hydrocarbons has been examined. A rapid assay method for detection of metabolites derived from 5α-[4-C]cholestane is described. Out of 87 organisms tested, 16 gave isolable oxidation products, while others degraded the hydrocarbon without producing stable intermediates. Preliminary characterization of these compounds is based on chromatographic properties.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-126-2-267
1981-10-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/126/2/mic-126-2-267.html?itemId=/content/journal/micro/10.1099/00221287-126-2-267&mimeType=html&fmt=ahah

References

  1. Bell A. M., Denny W. A., Jones E. R. H., Meakins G. D., Muller W. E. 1972a; Microbiological hydroxylation of steroids, V. Journal of the Chemical Society (Perkin I)2759–2765
    [Google Scholar]
  2. Bell A. M., Cherry K., Clark I. M., Denny W. A., Jones E. R. H., Meakins G. D., Woodgate P. D. 1972b; Microbiological hydroxylation of steroids. IV. Journal of the Chemical Society (Perkin I)2081–2095
    [Google Scholar]
  3. Brown R. L., Peterson G. E. 1966; Cholesterol oxidation by soil actinomycetes. Journal of General Microbiology 45:441–450
    [Google Scholar]
  4. Conner R. L., Mallory F. B., Landrey J. R., Iyengar C. W. L. 1969; Conversion of cholesterol to Δ5,7,22-cholestatrien-3β-ol by Tetra-hymena pyriformis. . Journal of Biological Chemistry 244:2325–2333
    [Google Scholar]
  5. Enzell C. R. 1966; Deuterium labelling by Clemmensen reduction. Tetrahedron Letter1285–1288
    [Google Scholar]
  6. Galu-Keinle M., Varma K. R., Mulheirn L. J., Yagen B., Caspi E. 1973; Reduction of Δ24 of lanosterol in the biosynthesis of cholesterol. II. Stereochemistry of addition of the C-25 proton. Journal of the American Chemical Society 95:1996–2001
    [Google Scholar]
  7. Mcmorris T. C., Marksdale A. W. 1967; Isolation of a sex hormone from the water mould Achlya bisexualis. . Nature, London 215:320–321
    [Google Scholar]
  8. Marscheck W. J., Karim A. 1973; Preparation of metabolites of spironolactone by microbial oxygenation. Applied Microbiology 25:647–649
    [Google Scholar]
  9. Mulheirn L. J., Ryback G. 1975; Stereochemistry of some steranes from geological sources. Nature, London 256:301–302
    [Google Scholar]
  10. Mulheirn L. J., Aberhart D. J., Caspi E. 1971; Dehydrogenation of sterols by the protozoan Tetrahymena pyriformis. . Journal of Biological Chemistry 246:6556–6559
    [Google Scholar]
  11. Ryback G. 1976; Chromatography of saturated steroid hydrocarbons (steranes) on alumina. Journal of Chromatography 116:207–210
    [Google Scholar]
  12. Schubert K., Ritter F., Sorkina T., Boehme K. H., Hoarhold C. 1969; Catabolism of steroids VIII. Journal of Steroid Biochemistry 1:1–7
    [Google Scholar]
  13. Sih C. J., Wang K. C., Tai H. H. 1968a; Mechanism of steroid oxidation by micro-organisms. XIII. C22 acid intermediates in the degradation of the cholesterol sidechain. Biochemistry 7:796–807
    [Google Scholar]
  14. Sih C. J., Tai H. H., Lee S. S., Coombe R. G. 1968b; Mechanisms of steroid oxidation by micro-organisms. XIV. Pathway of cholesterol sidechain degradation. Biochemistry 7:808–818
    [Google Scholar]
  15. Zaretskaya I. I., Kogan L. M., Tikhomivora O. B., Sis J. D., Wulfson N. S., Zaretskii V. I., Zaiken V. G., Skyrabin G. R., Torgov I. V. 1968; Microbial hydroxylation of the cholesterol sidechain. Tetrahedron 24:1595–1600
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-126-2-267
Loading
/content/journal/micro/10.1099/00221287-126-2-267
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error