Simultaneous Assay of Dihydroxyacetone Synthase and Transketolase in a Methylotrophic Yeast Grown in Continuous Culture. A Cautionary Note

References

1. Cooper J., Srere P. A., Tabachnick M., Racker E. 1958; The oxidative pentose phosphate cycle. II. Quantitative determination of intermediates and enzymes. Archives of Biochemistry and Biophysics 74:306–314
   [Google Scholar]
2. Dawson R. M. C., Elliott D. C., Elliott W. H., Jones K. M. 1969 Data for Biochemical Research, 2nd. pp. 615–616 Oxford: Clarendon Press.;
   [Google Scholar]
3. Egli T., van Dijken J. P., Veenhuis M., Harder W., Fiechter A. 1980; Methanol metabolism in yeasts: regulation of the synthesis of catabolic enzymes. Archives of Microbiology 124:115–121
   [Google Scholar]
4. Kato N., Nishizawa T., Sakazawa C., Tani Y., Yamada H. 1979; Xylulose 5-phosphate dependent fixation of formaldehyde in a methanol-utilizing yeast Kloeckera sp. no. 2201. Agricultural and Biological Chemistry 43:2013–2015
   [Google Scholar]
5. O’Connor M. L., Quayle J. R. 1980; Pentose phosphate-dependent fixation of formaldehyde by methanol-grown Hansenula polymorpha and Candida boidinii. Journal of General Microbiology 120:219–225
   [Google Scholar]
6. van Dijken J. P., Quayle J. R. 1977; Fructose metabolism in four Pseudomonas species. Archives of Microbiology 114:281–286
   [Google Scholar]
7. van Dijken J. P., Otto R., Harder W. 1976; Growth of Hansenula polymorpha in a methanol-limited chemostat. Physiological responses due to involvement of methanol oxidase as a key enzyme in methanol metabolism. Archives of Microbiology 111:137–144
   [Google Scholar]
8. van Dijken J. P., Harder W., Beardsmore A. J., Quayle J. R. 1978; Dihydroxy acetone: an intermediate in the assimilation of methanol by yeasts?. FEMS Microbiology Letters 4:97–102
   [Google Scholar]
9. Waites M. J., Quayle J. R. 1980; Dihydroxy acetone: a product of xylulose 5-phosphate-dependent fixation of formaldehyde by methanol-grown Candida boidinii. Journal of General Microbiology 118:321–327
   [Google Scholar]
10. Waites M. J., Quayle J. R. 1981; The interrelation between transketolase and dihydroxy acetone synthase activities in the methylotrophic yeast Candida boidinii. Journal of General Micro biology 124:309–316
    [Google Scholar]
11. Waites M. J., Lindley N. D., Quayle J. R. 1981; Determination of the labelling pattern of dihydroxyacetone and hexose phosphate following a brief incubation of methanol-grown Hansenula polymorpha with [¹⁴C]methanol. Journal of General Microbiology 122:193–199
    [Google Scholar]
12. Wood T. 1970; Spectrophotometric assay for d-ribose-5-phosphate ketol isomerase and for d-ribulose-5-phosphate 3-epimerase. Analytical Biochemistry 33:297–306
    [Google Scholar]